Abstract
Establishment of protective immunity depends critically on IFN-γ. We show that in human peripheral blood mononuclear cells, low doses of IL-2 greatly potentiate the response of the IFN-γ gene to mitogen, by over 100-fold. By itself, IL-2 is unable to induce IFN-γ mRNA to a significant extent. Yet, exposure to IL-2 leads to cellular commitment within a few hours, expressed by greatly enhanced accumulation of IFN-γ mRNA upon subsequent exposure to phytohemagglutinin. Changes induced by IL-2 do not relieve the requirement for de novo protein synthesis during the early phases of induction of IFN-γ gene expression. IL-2 may induce a component essential for induction of IFN-γ mRNA that is utilized during subsequent exposure to a mitogenic signal. Our results demonstrate synergy between IL-2 and mitogen in IFN-γ gene induction.
Original language | English |
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Pages (from-to) | 213-216 |
Number of pages | 4 |
Journal | Immunology Letters |
Volume | 44 |
Issue number | 2-3 |
DOIs | |
State | Published - 2 Jan 1995 |
Keywords
- Co-stimulatory factor
- Human interferon-γ Gene expression
- Interleukin-2
- Mitogenic induction