TY - JOUR
T1 - Intracerebral complement C5a receptor (CD88) expression is regulated by TNF and lymphotoxin-α following closed head injury in mice
AU - Stahel, Philip F.
AU - Kariya, Karin
AU - Shohami, Esther
AU - Barnum, Scott R.
AU - Eugster, Hans Pietro
AU - Trentz, Otmar
AU - Kossmann, Thomas
AU - Morganti-Kossmann, Maria C.
PY - 2000/9/22
Y1 - 2000/9/22
N2 - The anaphylatoxin C5a is a potent mediator of inflammation in the CNS. We analyzed the intracerebral expression of the C5a receptor (C5aR) in a model of closed head injury (CHI) in mice. Up-regulation of C5aR mRNA and protein expression was observed mainly on neurons in sham-operated and head- injured wild-type mice at 24 h. In contrast, in TNF/lymphotoxin-α knockout mice, the intracerebral C5aR expression remained at low constitutive levels after sham operation, whereas it strongly increased in response to trauma between 24 and 72 h. Interestingly, by 7 days after CHI, the intrathecal C5aR expression was clearly attenuated in the knockout animals. These data show that the posttraumatic neuronal expression of the C5aR is, at least in part, regulated by TNF and lymphotoxin-α at 7 days after trauma. (C) 2000 Elsevier Science B.V.
AB - The anaphylatoxin C5a is a potent mediator of inflammation in the CNS. We analyzed the intracerebral expression of the C5a receptor (C5aR) in a model of closed head injury (CHI) in mice. Up-regulation of C5aR mRNA and protein expression was observed mainly on neurons in sham-operated and head- injured wild-type mice at 24 h. In contrast, in TNF/lymphotoxin-α knockout mice, the intracerebral C5aR expression remained at low constitutive levels after sham operation, whereas it strongly increased in response to trauma between 24 and 72 h. Interestingly, by 7 days after CHI, the intrathecal C5aR expression was clearly attenuated in the knockout animals. These data show that the posttraumatic neuronal expression of the C5aR is, at least in part, regulated by TNF and lymphotoxin-α at 7 days after trauma. (C) 2000 Elsevier Science B.V.
KW - C5a
KW - Complement
KW - Knockout mice
KW - Serpentine receptors
KW - Traumatic brain injury
UR - http://www.scopus.com/inward/record.url?scp=0034703412&partnerID=8YFLogxK
U2 - 10.1016/S0165-5728(00)00304-0
DO - 10.1016/S0165-5728(00)00304-0
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C2 - 10996218
AN - SCOPUS:0034703412
SN - 0165-5728
VL - 109
SP - 164
EP - 172
JO - Journal of Neuroimmunology
JF - Journal of Neuroimmunology
IS - 2
ER -