TY - JOUR
T1 - Intramuscular mRNA BNT162b2 vaccine against SARS-CoV-2 induces neutralizing salivary IgA
AU - Stolovich-Rain, Miri
AU - Kumari, Sujata
AU - Friedman, Ahuva
AU - Kirillov, Saveliy
AU - Socol, Yakov
AU - Billan, Maria
AU - Pal, Ritesh Ranjan
AU - Das, Kathakali
AU - Golding, Peretz
AU - Oiknine-Djian, Esther
AU - Sirhan, Salim
AU - Bejerano-Sagie, Michal
AU - Cohen-Kfir, Einav
AU - Gold, Naama
AU - Fahoum, Jamal
AU - Kumar, Manoj
AU - Elgrably-Weiss, Maya
AU - Zhou, Bing
AU - Ravins, Miriam
AU - Gatt, Yair E.
AU - Bhattacharya, Saurabh
AU - Zelig, Orly
AU - Wiener, Reuven
AU - Wolf, Dana G.
AU - Elinav, Hila
AU - Strahilevitz, Jacob
AU - Padawer, Dan
AU - Baraz, Leah
AU - Rouvinski, Alexander
N1 - Publisher Copyright:
Copyright © 2023 Stolovich-Rain, Kumari, Friedman, Kirillov, Socol, Billan, Pal, Das, Golding, Oiknine-Djian, Sirhan, Sagie, Cohen-Kfir, Gold, Fahoum, Kumar, Elgrably-Weiss, Zhou, Ravins, Gatt, Bhattacharya, Zelig, Wiener, Wolf, Elinav, Strahilevitz, Padawer, Baraz and Rouvinski.
PY - 2023/1/30
Y1 - 2023/1/30
N2 - Intramuscularly administered vaccines stimulate robust serum neutralizing antibodies, yet they are often less competent in eliciting sustainable “sterilizing immunity” at the mucosal level. Our study uncovers a strong temporary neutralizing mucosal component of immunity, emanating from intramuscular administration of an mRNA vaccine. We show that saliva of BNT162b2 vaccinees contains temporary IgA targeting the receptor-binding domain (RBD) of severe acute respiratory syndrome coronavirus-2 spike protein and demonstrate that these IgAs mediate neutralization. RBD-targeting IgAs were found to associate with the secretory component, indicating their bona fide transcytotic origin and their polymeric multivalent nature. The mechanistic understanding of the high neutralizing activity provided by mucosal IgA, acting at the first line of defense, will advance vaccination design and surveillance principles and may point to novel treatment approaches and new routes of vaccine administration and boosting.
AB - Intramuscularly administered vaccines stimulate robust serum neutralizing antibodies, yet they are often less competent in eliciting sustainable “sterilizing immunity” at the mucosal level. Our study uncovers a strong temporary neutralizing mucosal component of immunity, emanating from intramuscular administration of an mRNA vaccine. We show that saliva of BNT162b2 vaccinees contains temporary IgA targeting the receptor-binding domain (RBD) of severe acute respiratory syndrome coronavirus-2 spike protein and demonstrate that these IgAs mediate neutralization. RBD-targeting IgAs were found to associate with the secretory component, indicating their bona fide transcytotic origin and their polymeric multivalent nature. The mechanistic understanding of the high neutralizing activity provided by mucosal IgA, acting at the first line of defense, will advance vaccination design and surveillance principles and may point to novel treatment approaches and new routes of vaccine administration and boosting.
KW - BNT162b2 vaccine
KW - SARS-CoV-2 neutralizing Abs
KW - mucosal immunity
KW - secretory IgA
KW - secretory component
UR - http://www.scopus.com/inward/record.url?scp=85148259517&partnerID=8YFLogxK
U2 - 10.3389/fimmu.2022.933347
DO - 10.3389/fimmu.2022.933347
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C2 - 36798518
AN - SCOPUS:85148259517
SN - 1664-3224
VL - 13
JO - Frontiers in Immunology
JF - Frontiers in Immunology
M1 - 933347
ER -
