Involvement of protein synthesis in the post-translational control of glutamine synthetase activity of cultured hepatoma cells

A. Freikopf*, R. G. Kulka

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Glutamine accelerates the degradation of glutamine synthetase in hepatoma tissue culture (HTC) cells. Preincubation of HTC cells in low glutamine with cycloheximide or emetine for 30 min prevents the decay of glutamine synthetase activity elicited by adding high concentrations of glutamine. However, when cycloheximide or emetine are added with or after high concentrations of glutamine the decay of glutamine synthetase activity is not immediately inhibited but proceeds for 1-3 hr. These observations suggest that the modulation of glutamine synthetase activity by glutamine involves a labile polypeptide factor which is synthesised both in the presence and absence of glutamine and is stabilized by glutamine.

Original languageEnglish
Pages (from-to)1195-1200
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume72
Issue number3
DOIs
StatePublished - 4 Oct 1976

Fingerprint

Dive into the research topics of 'Involvement of protein synthesis in the post-translational control of glutamine synthetase activity of cultured hepatoma cells'. Together they form a unique fingerprint.

Cite this