Abstract
Glutamine accelerates the degradation of glutamine synthetase in hepatoma tissue culture (HTC) cells. Preincubation of HTC cells in low glutamine with cycloheximide or emetine for 30 min prevents the decay of glutamine synthetase activity elicited by adding high concentrations of glutamine. However, when cycloheximide or emetine are added with or after high concentrations of glutamine the decay of glutamine synthetase activity is not immediately inhibited but proceeds for 1-3 hr. These observations suggest that the modulation of glutamine synthetase activity by glutamine involves a labile polypeptide factor which is synthesised both in the presence and absence of glutamine and is stabilized by glutamine.
Original language | English |
---|---|
Pages (from-to) | 1195-1200 |
Number of pages | 6 |
Journal | Biochemical and Biophysical Research Communications |
Volume | 72 |
Issue number | 3 |
DOIs | |
State | Published - 4 Oct 1976 |