TY - JOUR
T1 - Involvement of Sir2/4 in silencing of DNA breakage and recombination on mouse YACs during yeast meiosis
AU - Klieger, Yair
AU - Yizhar, Ofer
AU - Zenvirth, Drora
AU - Shtepel-Milman, Neta
AU - Snoek, Margriet
AU - Simchen, Giora
PY - 2005/3
Y1 - 2005/3
N2 - Yeast artificial chromosomes (YACs) that contain human DNA backbone undergo DNA double-strand breaks (DSBs) and recombination during yeast meiosis at rates similar to the yeast native chromosomes. Surprisingly, YACs containing DNA covering a recombination hot spot in the mouse major histocompatibility complex class III region do not show meiotic DSBs and undergo meiotic recombination at reduced levels. Moreover, segregation of these YACs during meiosis is seriously compromised. In meiotic yeast cells carrying the mutations sir2 or sir4, but not sir3, these YACs show DSBs, suggesting that a unique chromatin structure of the YACs, involving Sir2 and Sir4, protects the YACs from the meiotic recombination machinery. We speculate that the paucity of DSBs and recombination events on these YACs during yeast meiosis may reflect the refractory nature of the corresponding region in the mouse genome.
AB - Yeast artificial chromosomes (YACs) that contain human DNA backbone undergo DNA double-strand breaks (DSBs) and recombination during yeast meiosis at rates similar to the yeast native chromosomes. Surprisingly, YACs containing DNA covering a recombination hot spot in the mouse major histocompatibility complex class III region do not show meiotic DSBs and undergo meiotic recombination at reduced levels. Moreover, segregation of these YACs during meiosis is seriously compromised. In meiotic yeast cells carrying the mutations sir2 or sir4, but not sir3, these YACs show DSBs, suggesting that a unique chromatin structure of the YACs, involving Sir2 and Sir4, protects the YACs from the meiotic recombination machinery. We speculate that the paucity of DSBs and recombination events on these YACs during yeast meiosis may reflect the refractory nature of the corresponding region in the mouse genome.
UR - http://www.scopus.com/inward/record.url?scp=14844288963&partnerID=8YFLogxK
U2 - 10.1091/mbc.E04-07-0592
DO - 10.1091/mbc.E04-07-0592
M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???
C2 - 15647382
AN - SCOPUS:14844288963
SN - 1059-1524
VL - 16
SP - 1449
EP - 1455
JO - Molecular Biology of the Cell
JF - Molecular Biology of the Cell
IS - 3
ER -