Iron chelators of the pyridoxal 2-pyridyl hydrazone class. part III.1 Ionisation and conformational characteristics of the ligands

Prayong Doungdee*, Shalom Sarel, Israel Ringel, Dan Gibson, Nipon Wongvisetsirikul, Shelly Avramovici-Grisaru

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

pKa values of three biologically active iron chelators: pyridoxal 2-pyridyl hydrazone (PPH), 1-[N-methylpyridoxylidenium]-2-[2′-pyridyl]hydrazineiodide (MPH), 1-[N-elhoxycar-bonylmethylpyridoxylidenium]-2-[2′-pyridyl]hydrazine bromide (EPH) have been determined by a combination of ab initio calculations and pH-dependence of 13C nmr spectroscopy. In conformity with pyridoxal isonicotinoyl hydrazone (PIH), all ligands included in this study the pKa values invariably increase in the ordering: pyridinium protonation < pyridoxylidenium protonation < phenolate protonation < amine-hydrazone protonation < alkoxide protonation. Identical ordering was obtained by ab initio calculations, based on STO-3G set. Mulliken population analysis indicates that the conformer of the lowest energy of PPH, (I), contains an internal 6-membered-ring H-bond. Rotation about C3 - C8 bond in (I), to yield conformer (IV), requires 8.8 kcal/mol, whereas its internal H-bonding (I → II) accounts for 5.8 kcal/mol. Protonation of (I) lowers significantly energies both of I → V (6.5 kcal), and I → VI (2.5 kcal) transitions.

Original languageEnglish
Pages (from-to)241-248
Number of pages8
JournalHeterocycles
Volume40
Issue number1
DOIs
StatePublished - 1 Jan 1995

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