Isachenkonia alkalipeptolytica gen. Nov. sp. nov., a new anaerobic, alkaliphilic proteolytic bacterium capable of reducing fe(III) and sulfur

Daria G. Zavarzina*, Tatyana N. Zhilina, Nadegda A. Kostrikina, Stepan V. Toshchakov, Ilya V. Kublanov

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

An obligately alkaliphilic, anaerobic, proteolytic bacterium was isolated from a sample of Tanatar III soda lake sediment (Altai region, Russia) and designated as strain Z-1701T. Cells of strain Z-1701T were short, straight, motile Gram-stain-positive rods. Growth of Z-1701T obligately depended on the presence of sodium carbonate. Strain Z-1701T could utilize various peptides mix-tures, such as beef and yeast extracts, peptone, soytone, trypticase and tryptone, as well as such proteins as albumin, gelatin and sodium caseinate. It was able to grow oligotrophically with 0.02 g l−1 yeast extract as the sole energy and carbon source. Carbohydrates did not support the growth of strain Z-1701T. The main products released during the growth of strain Z-1701T on tryptone were formate, acetate and ammonium. Strain Z-1701T was able to reduce ferrihydrite, Fe(III)-EDTA, anthraquinone-2,6-disulfonate and elemental sulfur, using proteinaceous substrates as electron donors. In all cases the presence of the electron acceptor in the medium stimulated growth. The main cellular fatty acids were iso-C15:0, iso-C15:0 aldehyde, iso-C15:1 ω6, C16:0, iso-C17:0 aldehyde, C16:0 aldehyde and C14:0. The DNA G+C content of the isolate was 43.9 mol%. Phylogenetic analysis based on the concatenated alignment of 120 protein-marker sequences revealed that strain Z-1701T falls into a cluster with the genus Tin-dallia, family Clostridiaceae. 16S rRNA gene sequence identity between strain Z-1701T and Tindallia species were 88.3–89.75%. On the basis of its phenotypic characteristics and phylogenetic position, the novel isolate is considered to be a representative of a novel genus and species for which the name Isachenkonia alkalipeptolytica gen. nov., sp. nov. is proposed, with Z-1701T (=JCM 32929Т=DSM 109060Т=VKM B-3261Т) as its type strain.

Original languageEnglish
Pages (from-to)4730-4738
Number of pages9
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume70
Issue number8
DOIs
StatePublished - 2020
Externally publishedYes

Bibliographical note

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© 2020, Microbiology Society. All rights reserved.

Keywords

  • Hypersaline soda lakes
  • Iron reduction
  • Isachenkonia
  • Natronophilic anaerobes
  • Proteolytic
  • Sulfidogens

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