Isolation and characterization of low- vs. High-density neutrophils in cancer

Jitka Y. Sagiv, Sandra Voels, Zvi Granot*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

28 Scopus citations

Abstract

Neutrophils are the most abundant of all white blood cells in the human circulation and serve as the first line of defense against microbial infections. Traditionally, neutrophils were viewed as a homogeneous population of myeloid cells. However, in recent years accumulating evidence has suggested that neutrophils are heterogeneous and that distinct neutrophil subsets may play very different roles. Here, we describe the methodology for isolation of high- and low-density neutrophils from the murine and human circulation using a density gradient and antibody based enrichment. We further describe the methodology for functional characterization of these different neutrophil subsets in the context of cancer.

Original languageAmerican English
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages179-193
Number of pages15
DOIs
StatePublished - 2016

Publication series

NameMethods in Molecular Biology
Volume1458
ISSN (Print)1064-3745

Bibliographical note

Publisher Copyright:
© Springer Science+Business Media New York 2016.

Keywords

  • Cancer
  • Cytotoxicity
  • Density
  • Enrichment
  • Flow cytometry
  • Isolation
  • Neutrophils

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