Isolation and characterization of streptococcus mutans phage as a possible treatment agent for caries

Hadar Ben-Zaken; Reut Kraitman; Shunit Coppenhagen-Glazer; Leron Khalifa; Sivan Alkalay-Oren; Daniel Gelman; Gilad Ben-Gal; Nurit Beyth; Ronen Hazan

doi:10.3390/v13050825

Isolation and characterization of streptococcus mutans phage as a possible treatment agent for caries

Hadar Ben-Zaken, Reut Kraitman, Shunit Coppenhagen-Glazer, Leron Khalifa, Sivan Alkalay-Oren, Daniel Gelman, Gilad Ben-Gal, Nurit Beyth, Ronen Hazan^*

^*Corresponding author for this work

Institute of Biomedical Research at the Faculty of Dental Medicine

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Streptococcus mutans is a key bacterium in dental caries, one of the most prevalent chronic infectious diseases. Conventional treatment fails to specifically target the pathogenic bacteria, while tending to eradicate commensal bacteria. Thus, caries remains one of the most common and challenging diseases. Phage therapy, which involves the use of bacterial viruses as anti-bacterial agents, has been gaining interest worldwide. Nevertheless, to date, only a few phages have been isolated against S. mutans. In this study, we describe the isolation and characterization of a new S. mutans phage, termed SMHBZ8, from hundreds of human saliva samples that were collected, filtered, and screened. The SMHBZ8 genome was sequenced and analyzed, visualized by TEM, and its antibacterial properties were evaluated in various states. In addition, we tested the lytic efficacy of SMHBZ8 against S. mutans in a human cariogenic dentin model. The isolation and characterization of SMHBZ8 may be the first step towards developing a potential phage therapy for dental caries.

Original language	American English
Article number	825
Journal	Viruses
Volume	13
Issue number	5
DOIs	https://doi.org/10.3390/v13050825
State	Published - May 2021

Bibliographical note

Funding Information:
Funding: This research was supported by The Legacy Heritage Clinical Research Initiative of the Israel Science Foundation (grant No. 540/17) (NB), The “United States—Israel Binational Science Foundation (BSF)” grant #2017123, the Israel Science Foundation (ISF) IPMP grant #ISF1349/20, the Rosetrees Trust grant A2232, and the Milgrom Family Support Program and the Isidor Kabakoff grant (grant number 0365076). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.

Keywords

Bacteriophage
Biofilm
Dental caries
Phage therapy
S. mutans
S. mutans phage
mutans phage
bacteriophage
dental caries
S
phage therapy
biofilm
mutans

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.3390/v13050825

Cite this

@article{b8810a076bd1415fb582320eb16b4616,

title = "Isolation and characterization of streptococcus mutans phage as a possible treatment agent for caries",

abstract = "Streptococcus mutans is a key bacterium in dental caries, one of the most prevalent chronic infectious diseases. Conventional treatment fails to specifically target the pathogenic bacteria, while tending to eradicate commensal bacteria. Thus, caries remains one of the most common and challenging diseases. Phage therapy, which involves the use of bacterial viruses as anti-bacterial agents, has been gaining interest worldwide. Nevertheless, to date, only a few phages have been isolated against S. mutans. In this study, we describe the isolation and characterization of a new S. mutans phage, termed SMHBZ8, from hundreds of human saliva samples that were collected, filtered, and screened. The SMHBZ8 genome was sequenced and analyzed, visualized by TEM, and its antibacterial properties were evaluated in various states. In addition, we tested the lytic efficacy of SMHBZ8 against S. mutans in a human cariogenic dentin model. The isolation and characterization of SMHBZ8 may be the first step towards developing a potential phage therapy for dental caries.",

keywords = "Bacteriophage, Biofilm, Dental caries, Phage therapy, S. mutans, S. mutans phage, mutans phage, bacteriophage, dental caries, S, phage therapy, biofilm, mutans",

author = "Hadar Ben-Zaken and Reut Kraitman and Shunit Coppenhagen-Glazer and Leron Khalifa and Sivan Alkalay-Oren and Daniel Gelman and Gilad Ben-Gal and Nurit Beyth and Ronen Hazan",

note = "Funding Information: Funding: This research was supported by The Legacy Heritage Clinical Research Initiative of the Israel Science Foundation (grant No. 540/17) (NB), The “United States—Israel Binational Science Foundation (BSF)” grant #2017123, the Israel Science Foundation (ISF) IPMP grant #ISF1349/20, the Rosetrees Trust grant A2232, and the Milgrom Family Support Program and the Isidor Kabakoff grant (grant number 0365076). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Publisher Copyright: {\textcopyright} 2021 by the authors. Licensee MDPI, Basel, Switzerland.",

year = "2021",

month = may,

doi = "10.3390/v13050825",

language = "American English",

volume = "13",

journal = "Viruses",

issn = "1999-4915",

publisher = "MDPI Multidisciplinary Digital Publishing Institute",

number = "5",

}

TY - JOUR

T1 - Isolation and characterization of streptococcus mutans phage as a possible treatment agent for caries

AU - Ben-Zaken, Hadar

AU - Kraitman, Reut

AU - Coppenhagen-Glazer, Shunit

AU - Khalifa, Leron

AU - Alkalay-Oren, Sivan

AU - Gelman, Daniel

AU - Ben-Gal, Gilad

AU - Beyth, Nurit

AU - Hazan, Ronen

N1 - Funding Information: Funding: This research was supported by The Legacy Heritage Clinical Research Initiative of the Israel Science Foundation (grant No. 540/17) (NB), The “United States—Israel Binational Science Foundation (BSF)” grant #2017123, the Israel Science Foundation (ISF) IPMP grant #ISF1349/20, the Rosetrees Trust grant A2232, and the Milgrom Family Support Program and the Isidor Kabakoff grant (grant number 0365076). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.

PY - 2021/5

Y1 - 2021/5

N2 - Streptococcus mutans is a key bacterium in dental caries, one of the most prevalent chronic infectious diseases. Conventional treatment fails to specifically target the pathogenic bacteria, while tending to eradicate commensal bacteria. Thus, caries remains one of the most common and challenging diseases. Phage therapy, which involves the use of bacterial viruses as anti-bacterial agents, has been gaining interest worldwide. Nevertheless, to date, only a few phages have been isolated against S. mutans. In this study, we describe the isolation and characterization of a new S. mutans phage, termed SMHBZ8, from hundreds of human saliva samples that were collected, filtered, and screened. The SMHBZ8 genome was sequenced and analyzed, visualized by TEM, and its antibacterial properties were evaluated in various states. In addition, we tested the lytic efficacy of SMHBZ8 against S. mutans in a human cariogenic dentin model. The isolation and characterization of SMHBZ8 may be the first step towards developing a potential phage therapy for dental caries.

AB - Streptococcus mutans is a key bacterium in dental caries, one of the most prevalent chronic infectious diseases. Conventional treatment fails to specifically target the pathogenic bacteria, while tending to eradicate commensal bacteria. Thus, caries remains one of the most common and challenging diseases. Phage therapy, which involves the use of bacterial viruses as anti-bacterial agents, has been gaining interest worldwide. Nevertheless, to date, only a few phages have been isolated against S. mutans. In this study, we describe the isolation and characterization of a new S. mutans phage, termed SMHBZ8, from hundreds of human saliva samples that were collected, filtered, and screened. The SMHBZ8 genome was sequenced and analyzed, visualized by TEM, and its antibacterial properties were evaluated in various states. In addition, we tested the lytic efficacy of SMHBZ8 against S. mutans in a human cariogenic dentin model. The isolation and characterization of SMHBZ8 may be the first step towards developing a potential phage therapy for dental caries.

KW - Bacteriophage

KW - Biofilm

KW - Dental caries

KW - Phage therapy

KW - S. mutans

KW - S. mutans phage

KW - mutans phage

KW - bacteriophage

KW - dental caries

KW - S

KW - phage therapy

KW - biofilm

KW - mutans

UR - http://www.scopus.com/inward/record.url?scp=85106177177&partnerID=8YFLogxK

U2 - 10.3390/v13050825

DO - 10.3390/v13050825

M3 - Article

C2 - 34063251

AN - SCOPUS:85106177177

SN - 1999-4915

VL - 13

JO - Viruses

JF - Viruses

IS - 5

M1 - 825

ER -

Isolation and characterization of streptococcus mutans phage as a possible treatment agent for caries

Abstract

Bibliographical note

Keywords

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this