TY - JOUR
T1 - Isolation and characterization of streptococcus mutans phage as a possible treatment agent for caries
AU - Ben-Zaken, Hadar
AU - Kraitman, Reut
AU - Coppenhagen-Glazer, Shunit
AU - Khalifa, Leron
AU - Alkalay-Oren, Sivan
AU - Gelman, Daniel
AU - Ben-Gal, Gilad
AU - Beyth, Nurit
AU - Hazan, Ronen
N1 - Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2021/5/2
Y1 - 2021/5/2
N2 - Streptococcus mutans is a key bacterium in dental caries, one of the most prevalent chronic infectious diseases. Conventional treatment fails to specifically target the pathogenic bacteria, while tending to eradicate commensal bacteria. Thus, caries remains one of the most common and challenging diseases. Phage therapy, which involves the use of bacterial viruses as anti-bacterial agents, has been gaining interest worldwide. Nevertheless, to date, only a few phages have been isolated against S. mutans. In this study, we describe the isolation and characterization of a new S. mutans phage, termed SMHBZ8, from hundreds of human saliva samples that were collected, filtered, and screened. The SMHBZ8 genome was sequenced and analyzed, visualized by TEM, and its antibacterial properties were evaluated in various states. In addition, we tested the lytic efficacy of SMHBZ8 against S. mutans in a human cariogenic dentin model. The isolation and characterization of SMHBZ8 may be the first step towards developing a potential phage therapy for dental caries.
AB - Streptococcus mutans is a key bacterium in dental caries, one of the most prevalent chronic infectious diseases. Conventional treatment fails to specifically target the pathogenic bacteria, while tending to eradicate commensal bacteria. Thus, caries remains one of the most common and challenging diseases. Phage therapy, which involves the use of bacterial viruses as anti-bacterial agents, has been gaining interest worldwide. Nevertheless, to date, only a few phages have been isolated against S. mutans. In this study, we describe the isolation and characterization of a new S. mutans phage, termed SMHBZ8, from hundreds of human saliva samples that were collected, filtered, and screened. The SMHBZ8 genome was sequenced and analyzed, visualized by TEM, and its antibacterial properties were evaluated in various states. In addition, we tested the lytic efficacy of SMHBZ8 against S. mutans in a human cariogenic dentin model. The isolation and characterization of SMHBZ8 may be the first step towards developing a potential phage therapy for dental caries.
KW - Bacteriophage
KW - Biofilm
KW - Dental caries
KW - Phage therapy
KW - S. mutans
KW - S. mutans phage
UR - http://www.scopus.com/inward/record.url?scp=85106177177&partnerID=8YFLogxK
U2 - 10.3390/v13050825
DO - 10.3390/v13050825
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C2 - 34063251
AN - SCOPUS:85106177177
SN - 1999-4915
VL - 13
JO - Viruses
JF - Viruses
IS - 5
M1 - 825
ER -