Isolation and characterization of the plasma membrane by two-phase partitioning from the alkalophilic cyanobacterium spirulina platensis

Chang Hong Xu; Ali Nejidat; Shimshon Belkin; Sammy Boussiba

doi:10.1093/oxfordjournals.pcp.a078651

Isolation and characterization of the plasma membrane by two-phase partitioning from the alkalophilic cyanobacterium spirulina platensis

Chang Hong Xu, Ali Nejidat, Shimshon Belkin, Sammy Boussiba^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Partition in aqueous dextran-polyethylene glycol two-phase system was used to isolate the plasma membranes from the alkalophilic cyanobacterium Spirulina platensis. The upper phase contained a colorless membranes obtained in relatively short time, 3-4 h. This fraction had a different protein profile than that of the thylakoid fraction obtained in the lower phase. It did not contain cytochrome c-oxidase activity, but retained characteristic Mg²⁺-ATPase activity that is sensitive to vanadate, stimulated by K⁺, and has a pH optimum near 8.5. These data support our assumption that the upper phase of the gradient consist of the plasma membrane of S. platensis.

Original language	English
Pages (from-to)	737-741
Number of pages	5
Journal	Plant and Cell Physiology
Volume	35
Issue number	5
DOIs	https://doi.org/10.1093/oxfordjournals.pcp.a078651
State	Published - 1994
Externally published	Yes

Keywords

ATPase activity
Cyanobacteria
Phase partition
Plasma membrane
Spirulina platensis

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10.1093/oxfordjournals.pcp.a078651

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abstract = "Partition in aqueous dextran-polyethylene glycol two-phase system was used to isolate the plasma membranes from the alkalophilic cyanobacterium Spirulina platensis. The upper phase contained a colorless membranes obtained in relatively short time, 3-4 h. This fraction had a different protein profile than that of the thylakoid fraction obtained in the lower phase. It did not contain cytochrome c-oxidase activity, but retained characteristic Mg2+-ATPase activity that is sensitive to vanadate, stimulated by K+, and has a pH optimum near 8.5. These data support our assumption that the upper phase of the gradient consist of the plasma membrane of S. platensis.",

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AU - Boussiba, Sammy

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AB - Partition in aqueous dextran-polyethylene glycol two-phase system was used to isolate the plasma membranes from the alkalophilic cyanobacterium Spirulina platensis. The upper phase contained a colorless membranes obtained in relatively short time, 3-4 h. This fraction had a different protein profile than that of the thylakoid fraction obtained in the lower phase. It did not contain cytochrome c-oxidase activity, but retained characteristic Mg2+-ATPase activity that is sensitive to vanadate, stimulated by K+, and has a pH optimum near 8.5. These data support our assumption that the upper phase of the gradient consist of the plasma membrane of S. platensis.

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Isolation and characterization of the plasma membrane by two-phase partitioning from the alkalophilic cyanobacterium spirulina platensis

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