Isolation and HPLC separation of polyunsaturated species of rat brain acyl-CoA produced during decapitation - Ischemia

Joseph Deutsch, Stanley I. Rapoport, A. David Purdon

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Acyl-CoA is a crucial metabolic intermediate for the incorporation of fatty acid into membrane phospholipid. The rat brain long chain acyl-CoAs were isolated and quantitated with a procedure involving solid phase extraction with an oligonucleotide purification cartridge, followed by separation and quantitation by HPLC with special emphasis on resolution of the polyunsaturate acyl-CoAs. This procedure is uniquely suited to the rapid analysis of all molecular species of brain acyl-CoA including the polyunsaturate molecular species which are affected by ischemia. A selective 3-4 fold increase in arachidonoyl-CoA was found in rat brain after 3 min of ischemia when compared with microwaved brain. In contrast, the concentration of all other molecular species of acyl-CoA did not change over the time course of the ischemia.

Original languageEnglish
Pages (from-to)389-392
Number of pages4
JournalPhosphorus, Sulfur and Silicon and the Related Elements
Volume109
Issue number1-4
DOIs
StatePublished - 1996

Keywords

  • Acyl-CoA
  • Arachidonic acid
  • Brain ischemia
  • HPLC
  • Phospholipids

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