Isolation and partial characterization of plasmids found in three Halobacterium volcanii isolates

I. Rosenshine, M. Mevarech

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12 Scopus citations

Abstract

Three new isolates of Halobacterium volcanii were screened for the presence of plasmids. Each of the different isolates was found to contain one plasmid. These plasmids do not show any homology to each other, nor to the previously isolated plasmid pHV2. Partial restriction maps of these plasmids were determined. One of the plasmids contains chromosomal repetitive sequences as judged by the existence of homologous sequences in the chromosomal DNA of the three isolates. Using the protoplast fusion technique, we showed that at least one of the newly isolated plasmids is compatible with pHV2.

Original languageEnglish
Pages (from-to)92-95
Number of pages4
JournalCanadian Journal of Microbiology
Volume35
Issue number1
DOIs
StatePublished - 1989
Externally publishedYes

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