TY - JOUR
T1 - Isolation and quantitation of long-chain acyl-coenzyme A esters in brain tissue by solid-phase extraction
AU - Deutsch, Joseph
AU - Grange, Eric
AU - Rapoport, Stanley I.
AU - Purdon, A. David
PY - 1994/8/1
Y1 - 1994/8/1
N2 - Long-chain acyl-CoA's are important intermediates in fatty acid oxidation and phospholipid metabolism. For quantitative analysis of brain acyl-CoA's, and to avoid decomposition due to high brain acyl-CoA hydrolase activity, a fast and efficient analytical method was developed for isolation and determination of acyl-CoA's. The analysis includes solid-phase extraction by an oligonucleotide purification cartridge and HPLC measurements using a synthetic internal standard. Estimates of concentration in rat brain are oleoyl-CoA (11.0 nmol/g), palmitoyl-CoA (6.0 nmol/g), stearoyl-CoA (4.0 nmol/g), and linoleoyl- and arachidonoyl-CoA (2.0 nmol/g) for a total concentration of 23 nmol/g brain.
AB - Long-chain acyl-CoA's are important intermediates in fatty acid oxidation and phospholipid metabolism. For quantitative analysis of brain acyl-CoA's, and to avoid decomposition due to high brain acyl-CoA hydrolase activity, a fast and efficient analytical method was developed for isolation and determination of acyl-CoA's. The analysis includes solid-phase extraction by an oligonucleotide purification cartridge and HPLC measurements using a synthetic internal standard. Estimates of concentration in rat brain are oleoyl-CoA (11.0 nmol/g), palmitoyl-CoA (6.0 nmol/g), stearoyl-CoA (4.0 nmol/g), and linoleoyl- and arachidonoyl-CoA (2.0 nmol/g) for a total concentration of 23 nmol/g brain.
UR - http://www.scopus.com/inward/record.url?scp=0027930011&partnerID=8YFLogxK
U2 - 10.1006/abio.1994.1344
DO - 10.1006/abio.1994.1344
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AN - SCOPUS:0027930011
SN - 0003-2697
VL - 220
SP - 321
EP - 323
JO - Analytical Biochemistry
JF - Analytical Biochemistry
IS - 2
ER -