Isolation and sequence analysis of rep a from the incurable 90 MDA plasmid of Azospirillum Brasilense

A. Vande Broek*, Y. Okon, J. Vanderleyden

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

An open reading frame encoding a protein of 312 amino acids was identified in an essential region of the 90MDa plasmid of Azospirillum brasilense. The putative protein shows similarity with RepA proteins of plasmids of Sphingomonas aromacitovorans, Rhodoter-mus marinus, Chlorobium limicola and Bifidobacterium asteroides and with the RepA protein of the broad host range plasmid pSa. A putative helix-turn-helix motif was identified in the central part of the A. brasilense RepA protein. Analysis of the upstream and downstream region of the A. brasilense rep A gene revealed the presence of eight 15-bp repeats, which can possibly function as iterons.

Original languageEnglish
Pages (from-to)101-107
Number of pages7
JournalMitochondrial DNA
Volume11
Issue number1-2
DOIs
StatePublished - 2000

Keywords

  • 90 MDa plasmid
  • Azospirillum
  • iterons
  • rcpA
  • replication initiator protein

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