Abstract
We isolated and charaterized a new Escherichia coli gene, htpX. The htpX gene has been localized at min 40.3 on the chromosome. We determined its transcription and translation start site. htpX expresses a 32-kDa protein from a monocistronic transcript; expression of this protein is induced by temperature upshift. htpX is expressed from a σ32-dependent promoter and is thus part of the heat shock regulon. Cells carrying a htpX gene disruption grow well at all temperatures and under all conditions tested and have no apparent phenotype. However, cells which overexpress a truncated form of the protein display a higher rate of degradation of puromycyl peptides.
Original language | American English |
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Pages (from-to) | 2944-2953 |
Number of pages | 10 |
Journal | Journal of Bacteriology |
Volume | 173 |
Issue number | 9 |
DOIs | |
State | Published - 1991 |