Isolation, characterization, and sequence of an Escherichia coli heat shock gene, htpX

D. Kornitzer*, D. Teff, S. Altuvia, A. B. Oppenheim

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

53 Scopus citations

Abstract

We isolated and charaterized a new Escherichia coli gene, htpX. The htpX gene has been localized at min 40.3 on the chromosome. We determined its transcription and translation start site. htpX expresses a 32-kDa protein from a monocistronic transcript; expression of this protein is induced by temperature upshift. htpX is expressed from a σ32-dependent promoter and is thus part of the heat shock regulon. Cells carrying a htpX gene disruption grow well at all temperatures and under all conditions tested and have no apparent phenotype. However, cells which overexpress a truncated form of the protein display a higher rate of degradation of puromycyl peptides.

Original languageAmerican English
Pages (from-to)2944-2953
Number of pages10
JournalJournal of Bacteriology
Volume173
Issue number9
DOIs
StatePublished - 1991

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