This paper reports the isolation of two putative D2R promoters from grey mullet, one 5′ flanking and the other an intronic sequence immediately upstream of the first coding exon. Promoter activity of the intronic sequence was confirmed in vitro through functional analysis using luciferase as reporter gene. The functional characteristics of the region flanking the 5′-UTR is currently under investigation.
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Acknowledgements We acknowledge support to J. Nocilla-do from the Australia–Israel Scientific Exchange Foundation for the work done at The Hebrew University in Israel, and from The University of Queensland for the post-graduate scholarship. This project was funded by an Aquaculture Industry Development Initiative (AIDI) grant to A. Elizur. We sincerely thank H. Thaggard for maintaining the mullet fish stocks.