JMJD6 regulates splicing of its own gene resulting in alternatively spliced isoforms with different nuclear targets

Nikoleta Raguz*, Astrid Heim, Eden Engal, Juste Wesche, Juliane Merl-Pham, Stefanie M. Hauck, Steffen Erkelenz, Heiner Schaal, Olivier Bensaude, Alexander Wolf, Maayan Salton, Angelika Böttger*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Scopus citations


Jumonji-domain-containing protein 6 (JMJD6) is a Fe(II) and 2-oxogluterate (2OG) dependent oxygenase involved in gene regulation through post-translationally modifying nuclear proteins. It is highly expressed in many cancer types and linked to tumor progression and metastasis. Four alternatively-spliced jmjd6 transcripts were annotated. Here, we focus on the two most abundantly expressed ones, which we call jmjd6-2 and jmjd6-Ex5. TCGA SpliceSeq data revealed a significant decrease of jmjd6-Ex5 transcripts in patients and postmortem tissue of several tumors. The two protein isoforms are distinguished by their C-terminal sequences, which include a serinerich region (polyS-domain) in JMJD6-2 that is not present in JMJD6-Ex5. Immunoprecipitation followed by LC-MS/MS for JMJD6-Ex5 shows that different sets of proteins interact with JMJD6-2 and JMJD6-Ex5 with only a few overlaps. In particular, we found TFIIF-associating CTD phosphatase (FCP1), proteins of the survival of motor neurons (SMN) complex, heterogeneous nuclear ribonucleoproteins (hnRNPs) and upstream binding factor (UBF) to interact with JMJD6Ex5. Like JMJD6-2, both UBF and FCP1 comprise a polyS-domain. The polyS domain of JMJD6-2 might block the interaction with polyS-domains of other proteins. In contrast, JMJD6-2 interacts with many SR-like proteins with arginine/serine-rich (RS)-domains, including several splicing factors. In an HIV-based splicing reporter assay, co-expression of JMJD6-2 inhibited exon inclusion, whereas JMJD6-Ex5 did not have any effect. Furthermore, the silencing of jmjd6 by siRNAs favored jmjd6-Ex5 transcripts, suggesting that JMJD6 controls splicing of its own pre-mRNA. The distinct molecular properties of JMJD6-2 and JMJD6-Ex5 open a lead into the functional implications of the variations of their relative abundance in tumors.

Original languageAmerican English
Article number6618
Pages (from-to)1-15
Number of pages15
JournalInternational Journal of Molecular Sciences
Issue number18
StatePublished - 2 Sep 2020

Bibliographical note

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© 2020 by the authors. Licensee MDPI, Basel, Switzerland.


  • Hydroxylation
  • PolyS domain
  • Splicing


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