Juvenile dermatomyositis: Serial studies of circulating autoantibodies to a 56kD nuclear protein

G. Cambridge*, E. Ovadia, D. A. Isenberg, V. Dubowitz, J. Sperling, R. Sperling

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

In this study we report that circulating antibodies recognising a 56kD protein, which is a component of large nuclear ribonuclear particles, are commonly found in children with juvenile onset dermatomyositis (JDM). These autoantibodies, as detected by Western blotting, were present in over 90% (24/26) of sera from JDM patients, which exceeds the number of patients with adult onset myositis who express this antibody (up to 85%). In addition, they were not found in healthy controls. Serial bleeds taken during the course of the disease in eleven children with JDM enabled us to follow the titre anti- 56kD autoantibodies. Sera were also tested by indirect immunofluorescence for anti-nuclear antibodies (ANA) using Hep 2 cells as substrate. These studies revealed two distinct patient groups: Group 1 with anti-56kD antibody positive and ANA positive; and Group 2 with anti-56kD antibody positive and ANA negative. In Group 1 there was some correlation between disease activity and anti-56kD levels which was absent among patients in Group 2.

Original languageEnglish
Pages (from-to)451-457
Number of pages7
JournalClinical and Experimental Rheumatology
Volume12
Issue number4
StatePublished - 1994
Externally publishedYes

Keywords

  • dermatomyositis autoantibodies

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