Keratinase of an anaerobic thermophilic bacterium Thermoanaerobacter sp. Strain 1004-09 isolated from a hot spring in the Baikal rift zone

I. V. Kublanov, K. B. Tsiroulnikov, E. N. Kaliberda, L. D. Rumsh, T. Haertlé, E. A. Bonch-Osmolovskaya

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Abstract

A thermophilic anaerobic bacterial strain 1004-09 belonging to the genus Thermoanaerobacter and capable of growth on protein substrates such as albumin, gelatin, casein, and α- and β-keratins was isolated from the Urinskii hot spring (Barguzin river valley, Republic of Buryatia, Russia). A 150-kDa serine proteinase was revealed in the strain supernatant; it exhibited optimal activity at 60°C and pH 9.3 and was capable of keratin hydrolysis. A number of characteristics for the strain 1004-09 keratinase were established including activation by SDS and NaCl and residual activity (15% to the activity of the intact protein) in the presence of 10% ethanol and acetone.

Original languageEnglish
Pages (from-to)67-75
Number of pages9
JournalMicrobiology
Volume78
Issue number1
DOIs
StatePublished - Feb 2009
Externally publishedYes

Keywords

  • Hydrolytic microorganisms
  • Keratin
  • Protein hydrolysis
  • Proteinases
  • Thermophilic microorganisms

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