Abstract
A thermophilic anaerobic bacterial strain 1004-09 belonging to the genus Thermoanaerobacter and capable of growth on protein substrates such as albumin, gelatin, casein, and α- and β-keratins was isolated from the Urinskii hot spring (Barguzin river valley, Republic of Buryatia, Russia). A 150-kDa serine proteinase was revealed in the strain supernatant; it exhibited optimal activity at 60°C and pH 9.3 and was capable of keratin hydrolysis. A number of characteristics for the strain 1004-09 keratinase were established including activation by SDS and NaCl and residual activity (15% to the activity of the intact protein) in the presence of 10% ethanol and acetone.
Original language | English |
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Pages (from-to) | 67-75 |
Number of pages | 9 |
Journal | Microbiology |
Volume | 78 |
Issue number | 1 |
DOIs | |
State | Published - Feb 2009 |
Externally published | Yes |
Keywords
- Hydrolytic microorganisms
- Keratin
- Protein hydrolysis
- Proteinases
- Thermophilic microorganisms