Key features of cereal genome organization as revealed by the use of cytosine methylation-sensitive restriction endonucleases

Graham Moore*, Shahal Abbo, Wing Cheung, Tracie Foote, Mike Gale, Robert Koebner, Andrew Leitch, Ilia Leitch, Tracy Money, Patrick Stancombe, Masahiro Yano, Richard Flavell

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

64 Scopus citations

Abstract

Unlike mammalian genomes, cereal (Gramineae) genomes exhibit little suppression of CpG dinucleotides. In cereal genomes, however, most of the numerous potential recognition sites for CpG methylation-sensitive restriction enzymes are methylated. Analysis of cereal genomic libraries and of regions flanking genes indicates that unmethylated NotI sites are useful landmarks for regions containing genes/single-copy sequences. Studies of a rye chromosome arm indicate that its pericentromeric region has a reduced density of unmethylated NotI (and MluI) sites and therefore of genes. Unmethylated MluI and NruI sites are distributed nonrandomly in the genomes of wheat, barley, and rice. Analysis of the genomic blocks defined by these sites in wheat and barley indicates that they are most likely to have arisen by amplification. These observations form the basis of a proposed model for the organization and evolution of the wheat, barley, and rice genomes.

Original languageAmerican English
Pages (from-to)472-482
Number of pages11
JournalGenomics
Volume15
Issue number3
DOIs
StatePublished - Mar 1993
Externally publishedYes

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