TY - JOUR
T1 - Killing of burkitt-lymphoma-derived daudi cells by ultraviolet-inactivated vaccinia virus
AU - Grunwald-Beard, Leora
AU - Gamliel, Haim
AU - Parag, Gisia
AU - Vedantham, Suresh
AU - Zakay-Rones, Zichria
PY - 1991/11
Y1 - 1991/11
N2 - Interaction of active and UV-inactivated vaccinia virus at high multiplicity caused cytological changes and inhibition in cellular protein and DNA synthesis, thus arresting the multiplication of Burkitt-lymphomaderived Daudi cells and eventually killing the cells. Adsorption to the cells but the lack of penetration was evident by immunofluorescence, electron microscopy and [3H]thymidine-labeled virus incorporation. Viral DNA synthesis or virus replication was not demonstrated. Thus, it appears that the massive adsorption of viral particles, active or UV-inactivated, or possibly a "toxic" component that resides in the virion, damages the plasma membrane and may be responsible for killing the cells by a mechanism of lysis from without.
AB - Interaction of active and UV-inactivated vaccinia virus at high multiplicity caused cytological changes and inhibition in cellular protein and DNA synthesis, thus arresting the multiplication of Burkitt-lymphomaderived Daudi cells and eventually killing the cells. Adsorption to the cells but the lack of penetration was evident by immunofluorescence, electron microscopy and [3H]thymidine-labeled virus incorporation. Viral DNA synthesis or virus replication was not demonstrated. Thus, it appears that the massive adsorption of viral particles, active or UV-inactivated, or possibly a "toxic" component that resides in the virion, damages the plasma membrane and may be responsible for killing the cells by a mechanism of lysis from without.
KW - Cytotoxicity
KW - Daudi-Burkitt lymphoma cells
KW - UV-inactivated virus
KW - Vaccinia virus
UR - http://www.scopus.com/inward/record.url?scp=0025933244&partnerID=8YFLogxK
U2 - 10.1007/BF01613289
DO - 10.1007/BF01613289
M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???
C2 - 1744162
AN - SCOPUS:0025933244
SN - 0171-5216
VL - 117
SP - 561
EP - 567
JO - Journal of Cancer Research and Clinical Oncology
JF - Journal of Cancer Research and Clinical Oncology
IS - 6
ER -