TY - JOUR
T1 - Kinetic modeling of Sendai virus fusion with PC‐12 cells
T2 - Effect of pH and temperature on fusion and viral inactivation
AU - PEDROSO DE LIMA, Maria da Conceição
AU - RAMALHO‐SANTOS, João
AU - MARTINS, Maria de Fátima
AU - PATO DE CARVALHO, Arsélio
AU - BAIROS, Vasco
AU - NIR, Shlomo
PY - 1992/4
Y1 - 1992/4
N2 - We have studied the fusion activity of Sendai virus, a lipid‐enveloped paramyxovirus, towards a line of adherent cells designated PC‐12. Fusion was monitored by the dequenching of octadecylrhodamine, a fluorescent non‐exchangeable probe. The results were analysed with a mass action kinetic model which could explain and predict the kinetics of virus–cell fusion. When the temperature was lowered from 37°C to 25°C, a sharp inhibition of the fusion process was observed, probably reflecting a constraint in the movement of viral glycoproteins at low temperatures. The rate constants of adhesion and fusion were reduced 3.5‐fold and 7‐fold, respectively, as the temperature was lowered from 37°C to 25°C. The fusion process seemed essentially pH‐independent, unlike the case of liposomes and erythrocyte ghosts. Preincubation of the virus in the absence of target cell membranes at neutral and alkaline pH (37°C, 30 min) did not affect the fusion process. However, a similar preincubation of the virus at pH = 5.0 resulted in marked, though slow, inhibition in fusion with the fusion rate constant being reduced 8‐fold. Viral preincubation for 5 min in the same acidic conditions yielded a mild inhibition of fusogenic activity, while preincubation in the cold (4°C, 30 min) did not alter viral fusion activity. These acid‐induced inhibitory effects could not be fully reversed by further viral preincubation at pH = 7.4 (37°C, 30 min). Changes in internal pH as well as endocytic activity of PC‐12 cells had small effect on the fusion process, thus indicating that Sendai virus fuses primarily with the plasma membranes.
AB - We have studied the fusion activity of Sendai virus, a lipid‐enveloped paramyxovirus, towards a line of adherent cells designated PC‐12. Fusion was monitored by the dequenching of octadecylrhodamine, a fluorescent non‐exchangeable probe. The results were analysed with a mass action kinetic model which could explain and predict the kinetics of virus–cell fusion. When the temperature was lowered from 37°C to 25°C, a sharp inhibition of the fusion process was observed, probably reflecting a constraint in the movement of viral glycoproteins at low temperatures. The rate constants of adhesion and fusion were reduced 3.5‐fold and 7‐fold, respectively, as the temperature was lowered from 37°C to 25°C. The fusion process seemed essentially pH‐independent, unlike the case of liposomes and erythrocyte ghosts. Preincubation of the virus in the absence of target cell membranes at neutral and alkaline pH (37°C, 30 min) did not affect the fusion process. However, a similar preincubation of the virus at pH = 5.0 resulted in marked, though slow, inhibition in fusion with the fusion rate constant being reduced 8‐fold. Viral preincubation for 5 min in the same acidic conditions yielded a mild inhibition of fusogenic activity, while preincubation in the cold (4°C, 30 min) did not alter viral fusion activity. These acid‐induced inhibitory effects could not be fully reversed by further viral preincubation at pH = 7.4 (37°C, 30 min). Changes in internal pH as well as endocytic activity of PC‐12 cells had small effect on the fusion process, thus indicating that Sendai virus fuses primarily with the plasma membranes.
UR - http://www.scopus.com/inward/record.url?scp=0026611884&partnerID=8YFLogxK
U2 - 10.1111/j.1432-1033.1992.tb16766.x
DO - 10.1111/j.1432-1033.1992.tb16766.x
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C2 - 1313363
AN - SCOPUS:0026611884
SN - 0014-2956
VL - 205
SP - 181
EP - 186
JO - European Journal of Biochemistry
JF - European Journal of Biochemistry
IS - 1
ER -