TY - JOUR
T1 - Kinetic parameters of glucose efflux from human red blood cells under zero-trans conditions
AU - Karlish, S. J.D.
AU - Lieb, W. R.
AU - Ram, D.
AU - Stein, W. D.
PY - 1972/1/17
Y1 - 1972/1/17
N2 - 1. 1. Efflux of glucose from human red blood cells under zero-trans conditions has been measured at 20°. 2. 2. The values of the Km and vmax were found to be 25 ± 3 mM and 139 ± 11 mmoles/min per 1 isotonic cell water, respectively. 3. 3. These observations indicate that the conventional, symmetric carrier is not a valid model for glucose transport in human red blood cells. 4. 4. Our results are consistent, however, with a recently proposed model in which transport occurs by the internal transfer of glucose molecules across protein tetramers embedded in the plasma membrane.
AB - 1. 1. Efflux of glucose from human red blood cells under zero-trans conditions has been measured at 20°. 2. 2. The values of the Km and vmax were found to be 25 ± 3 mM and 139 ± 11 mmoles/min per 1 isotonic cell water, respectively. 3. 3. These observations indicate that the conventional, symmetric carrier is not a valid model for glucose transport in human red blood cells. 4. 4. Our results are consistent, however, with a recently proposed model in which transport occurs by the internal transfer of glucose molecules across protein tetramers embedded in the plasma membrane.
UR - http://www.scopus.com/inward/record.url?scp=0015513318&partnerID=8YFLogxK
U2 - 10.1016/0005-2736(72)90014-4
DO - 10.1016/0005-2736(72)90014-4
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C2 - 5010989
AN - SCOPUS:0015513318
SN - 0005-2736
VL - 255
SP - 126
EP - 132
JO - Biochimica et Biophysica Acta - Biomembranes
JF - Biochimica et Biophysica Acta - Biomembranes
IS - 1
ER -