Abstract
We have developed a kinetic technique, combining resonance Raman spectroscopy and variable-speed continuous flow methods, to study molecular dynamics of isolated sites in macromolecules. Kinetic resonance Raman spectra of the retinylidene chromophore of bacteriorhodopsin have been obtained and the dynamics of the deprotonation of the Schiff base linkage is discussed.
| Original language | English |
|---|---|
| Pages (from-to) | 1328-1330 |
| Number of pages | 3 |
| Journal | Science |
| Volume | 195 |
| Issue number | 4284 |
| DOIs | |
| State | Published - 1977 |
| Externally published | Yes |