Kinetic resonance raman spectroscopy: Dynamics of deprotonation of the schiff base of bacteriorhodopsin

Michael A. Marcus; Aaron Lewis

doi:10.1126/science.841330

Kinetic resonance raman spectroscopy: Dynamics of deprotonation of the schiff base of bacteriorhodopsin

Michael A. Marcus^*
, Aaron Lewis

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

85 Scopus citations

Abstract

We have developed a kinetic technique, combining resonance Raman spectroscopy and variable-speed continuous flow methods, to study molecular dynamics of isolated sites in macromolecules. Kinetic resonance Raman spectra of the retinylidene chromophore of bacteriorhodopsin have been obtained and the dynamics of the deprotonation of the Schiff base linkage is discussed.

Original language	English
Pages (from-to)	1328-1330
Number of pages	3
Journal	Science
Volume	195
Issue number	4284
DOIs	https://doi.org/10.1126/science.841330
State	Published - 1977
Externally published	Yes

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title = "Kinetic resonance raman spectroscopy: Dynamics of deprotonation of the schiff base of bacteriorhodopsin",

abstract = "We have developed a kinetic technique, combining resonance Raman spectroscopy and variable-speed continuous flow methods, to study molecular dynamics of isolated sites in macromolecules. Kinetic resonance Raman spectra of the retinylidene chromophore of bacteriorhodopsin have been obtained and the dynamics of the deprotonation of the Schiff base linkage is discussed.",

author = "Marcus, \{Michael A.\} and Aaron Lewis",

year = "1977",

doi = "10.1126/science.841330",

language = "אנגלית",

volume = "195",

pages = "1328--1330",

journal = "Science",

issn = "0036-8075",

publisher = "American Association for the Advancement of Science",

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T1 - Kinetic resonance raman spectroscopy

T2 - Dynamics of deprotonation of the schiff base of bacteriorhodopsin

AU - Marcus, Michael A.

AU - Lewis, Aaron

PY - 1977

Y1 - 1977

N2 - We have developed a kinetic technique, combining resonance Raman spectroscopy and variable-speed continuous flow methods, to study molecular dynamics of isolated sites in macromolecules. Kinetic resonance Raman spectra of the retinylidene chromophore of bacteriorhodopsin have been obtained and the dynamics of the deprotonation of the Schiff base linkage is discussed.

AB - We have developed a kinetic technique, combining resonance Raman spectroscopy and variable-speed continuous flow methods, to study molecular dynamics of isolated sites in macromolecules. Kinetic resonance Raman spectra of the retinylidene chromophore of bacteriorhodopsin have been obtained and the dynamics of the deprotonation of the Schiff base linkage is discussed.

UR - https://www.scopus.com/pages/publications/0017600051

U2 - 10.1126/science.841330

DO - 10.1126/science.841330

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C2 - 841330

AN - SCOPUS:0017600051

SN - 0036-8075

VL - 195

SP - 1328

EP - 1330

JO - Science

JF - Science

IS - 4284

ER -

Kinetic resonance raman spectroscopy: Dynamics of deprotonation of the schiff base of bacteriorhodopsin

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