Kinetics of influenza virus fusion with the endosomal and plasma membranes of cultured cells. Effect of temperature

I. Nunes-Correia, S. Nir, M. C. Pedroso De Lima*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

We performed a detailed kinetic analysis of influenza virus fusion with the endosomal and plasma membranes of Madin Darby canine kidney (MDCK) cells and provided a comparison of the kinetic parameters obtained for both cases at 20°C and 37°C. Using our mass action kinetic model, we determined that the fusion rate constant, f, for influenza virus with the endosomal membrane was 0.02 s-1 at 37°C and 0.0035 s-1 at 20°C. The analysis of the fusion kinetics of influenza virus with the plasma membrane yielded that the fusion rate constants were close to those deduced with the endosomal membrane. The systematic kinetic analysis performed in this study provides for the first time a biophysical support for studies on influenza virus-cell fusion where the acidic endosomal internal environment is simulated artificially by lowering the pH of the medium.

Original languageEnglish
Pages (from-to)21-26
Number of pages6
JournalJournal of Membrane Biology
Volume195
Issue number1
DOIs
StatePublished - 1 Sep 2003

Keywords

  • Endocytosis
  • Influenza virus
  • Kinetic analysis
  • MDCK cells
  • Membrane fusion

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