Kinetics of linear rouleaux formation studied by visual monitoring of red cell dynamic organization

G. Barshtein, D. Wajnblum, S. Yedgar*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

65 Scopus citations


Red blood cells (RBCs) in the presence of plasma proteins or other macromolecules may form aggregates, normally in rouleaux formations, which are dispersed with increasing blood flow. Experimental observations have suggested that the spontaneous aggregation process involves the formation of linear rouleaux (FLR) followed by formation of branched rouleaux networks. Theoretical models for the spontaneous rouleaux formation were formulated, taking into consideration that FLR may involve both 'polymerization,' i.e., interaction between two single RBCs (e + e) and the addition of a single RBC to the end of an existing rouleau (e + r), as well as 'condensation' between two rouleaux by end-to-end addition (r + r). The present study was undertaken to experimentally examine the theoretical models and their assumptions, by visual monitoring of the spontaneous FLR (from singly dispersed RBC) in plasma, in a narrow gap flow chamber. The results validate the theoretical model, showing that FLR involves both polymerization and condensation, and that the kinetic constants for the above three types of intercellular interactions are the same, i.e., k(ee) = k(er) = k(rr) = k, and for all tested hematocrits (0.625-6%) k < 0.13 ± 0.03 s-1.

Original languageAmerican English
Pages (from-to)2470-2474
Number of pages5
JournalBiophysical Journal
Issue number5
StatePublished - 2000

Bibliographical note

Funding Information:
This study was supported by grants to SY from the Israel Science Foundation (482/963), the Israel Ministry of Sciences (1460-1-99), the Hebrew University R and D Authority, and the “Ezvonot” Fund, and a grant to GB from the Israel Ministry of Health (4165).


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