Kinetoplast DNA heterogeneity among Leishmania infantum strains in central Israel and Palestine

Abedelmajeed Nasereddin; Kifaya Azmi; Charles L. Jaffe; Suheir Ereqat; Ahmad Amro; Samer Sawalhah; Gad Baneth; Gabriele Schönian; Ziad Abdeen

doi:10.1016/j.vetpar.2008.12.003

Kinetoplast DNA heterogeneity among Leishmania infantum strains in central Israel and Palestine

Abedelmajeed Nasereddin^*, Kifaya Azmi, Charles L. Jaffe, Suheir Ereqat, Ahmad Amro, Samer Sawalhah, Gad Baneth, Gabriele Schönian, Ziad Abdeen

^*Corresponding author for this work

The Koret School of Veterinary Medicine

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

Leishmania infantum is the causative agent of canine and human visceral leishmaniasis in Israel and Palestine. Amplification of the kinetoplast DNA (kDNA), followed by restriction fragment length polymorphism (RFLP) analysis with restriction enzymes was used to examine the genotypic association between L. infantum strains isolated from 22 dogs and 2 humans from these adjoining regions. Results showed wide kDNA heterogeneity in these strains. Two main clusters (A and B) were identified. Cluster A was restricted to central Israel and was mainly found in strains isolated after 2002 whereas cluster B included parasites from central Israel and the West Bank. The kDNA microheterogeneity in L. infantum parasite populations as shown by genotyping with the kDNA-PCR and RFLP provided a tool to study the epidemiology of the disease and track its spread in central Israel and Palestine.

Original language	English
Pages (from-to)	126-130
Number of pages	5
Journal	Veterinary Parasitology
Volume	161
Issue number	1-2
DOIs	https://doi.org/10.1016/j.vetpar.2008.12.003
State	Published - 6 Apr 2009

Bibliographical note

Funding Information:
This study was supported by the U.S. Middle East Regional Cooperation (MERC) Project NIH-NIAID Contract No. TA-MOU-03-M23-015. We thank L.F. Schnur for his dedicated help.

Keywords

Israel
Kinetoplast DNA
Leishmania infantum
Palestine
Polymerase chain reaction
Restriction fragment length polymorphism

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.vetpar.2008.12.003

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title = "Kinetoplast DNA heterogeneity among Leishmania infantum strains in central Israel and Palestine",

abstract = "Leishmania infantum is the causative agent of canine and human visceral leishmaniasis in Israel and Palestine. Amplification of the kinetoplast DNA (kDNA), followed by restriction fragment length polymorphism (RFLP) analysis with restriction enzymes was used to examine the genotypic association between L. infantum strains isolated from 22 dogs and 2 humans from these adjoining regions. Results showed wide kDNA heterogeneity in these strains. Two main clusters (A and B) were identified. Cluster A was restricted to central Israel and was mainly found in strains isolated after 2002 whereas cluster B included parasites from central Israel and the West Bank. The kDNA microheterogeneity in L. infantum parasite populations as shown by genotyping with the kDNA-PCR and RFLP provided a tool to study the epidemiology of the disease and track its spread in central Israel and Palestine.",

keywords = "Israel, Kinetoplast DNA, Leishmania infantum, Palestine, Polymerase chain reaction, Restriction fragment length polymorphism",

author = "Abedelmajeed Nasereddin and Kifaya Azmi and Jaffe, {Charles L.} and Suheir Ereqat and Ahmad Amro and Samer Sawalhah and Gad Baneth and Gabriele Sch{\"o}nian and Ziad Abdeen",

note = "Funding Information: This study was supported by the U.S. Middle East Regional Cooperation (MERC) Project NIH-NIAID Contract No. TA-MOU-03-M23-015. We thank L.F. Schnur for his dedicated help.",

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AU - Nasereddin, Abedelmajeed

AU - Azmi, Kifaya

AU - Jaffe, Charles L.

AU - Ereqat, Suheir

AU - Amro, Ahmad

AU - Sawalhah, Samer

AU - Baneth, Gad

AU - Schönian, Gabriele

AU - Abdeen, Ziad

N1 - Funding Information: This study was supported by the U.S. Middle East Regional Cooperation (MERC) Project NIH-NIAID Contract No. TA-MOU-03-M23-015. We thank L.F. Schnur for his dedicated help.

PY - 2009/4/6

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N2 - Leishmania infantum is the causative agent of canine and human visceral leishmaniasis in Israel and Palestine. Amplification of the kinetoplast DNA (kDNA), followed by restriction fragment length polymorphism (RFLP) analysis with restriction enzymes was used to examine the genotypic association between L. infantum strains isolated from 22 dogs and 2 humans from these adjoining regions. Results showed wide kDNA heterogeneity in these strains. Two main clusters (A and B) were identified. Cluster A was restricted to central Israel and was mainly found in strains isolated after 2002 whereas cluster B included parasites from central Israel and the West Bank. The kDNA microheterogeneity in L. infantum parasite populations as shown by genotyping with the kDNA-PCR and RFLP provided a tool to study the epidemiology of the disease and track its spread in central Israel and Palestine.

AB - Leishmania infantum is the causative agent of canine and human visceral leishmaniasis in Israel and Palestine. Amplification of the kinetoplast DNA (kDNA), followed by restriction fragment length polymorphism (RFLP) analysis with restriction enzymes was used to examine the genotypic association between L. infantum strains isolated from 22 dogs and 2 humans from these adjoining regions. Results showed wide kDNA heterogeneity in these strains. Two main clusters (A and B) were identified. Cluster A was restricted to central Israel and was mainly found in strains isolated after 2002 whereas cluster B included parasites from central Israel and the West Bank. The kDNA microheterogeneity in L. infantum parasite populations as shown by genotyping with the kDNA-PCR and RFLP provided a tool to study the epidemiology of the disease and track its spread in central Israel and Palestine.

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KW - Polymerase chain reaction

KW - Restriction fragment length polymorphism

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Kinetoplast DNA heterogeneity among Leishmania infantum strains in central Israel and Palestine

Abstract

Bibliographical note

Keywords

UN SDGs

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