Kinetoplast DNA heterogeneity among Leishmania infantum strains in central Israel and Palestine

Abedelmajeed Nasereddin*, Kifaya Azmi, Charles L. Jaffe, Suheir Ereqat, Ahmad Amro, Samer Sawalhah, Gad Baneth, Gabriele Schönian, Ziad Abdeen

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Leishmania infantum is the causative agent of canine and human visceral leishmaniasis in Israel and Palestine. Amplification of the kinetoplast DNA (kDNA), followed by restriction fragment length polymorphism (RFLP) analysis with restriction enzymes was used to examine the genotypic association between L. infantum strains isolated from 22 dogs and 2 humans from these adjoining regions. Results showed wide kDNA heterogeneity in these strains. Two main clusters (A and B) were identified. Cluster A was restricted to central Israel and was mainly found in strains isolated after 2002 whereas cluster B included parasites from central Israel and the West Bank. The kDNA microheterogeneity in L. infantum parasite populations as shown by genotyping with the kDNA-PCR and RFLP provided a tool to study the epidemiology of the disease and track its spread in central Israel and Palestine.

Original languageAmerican English
Pages (from-to)126-130
Number of pages5
JournalVeterinary Parasitology
Volume161
Issue number1-2
DOIs
StatePublished - 6 Apr 2009

Bibliographical note

Funding Information:
This study was supported by the U.S. Middle East Regional Cooperation (MERC) Project NIH-NIAID Contract No. TA-MOU-03-M23-015. We thank L.F. Schnur for his dedicated help.

Keywords

  • Israel
  • Kinetoplast DNA
  • Leishmania infantum
  • Palestine
  • Polymerase chain reaction
  • Restriction fragment length polymorphism

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