TY - JOUR
T1 - Kinetoplast DNA minicircles of trypanosomatids encode for a protein product
AU - Shlomai, Joseph
AU - Zadok, Anat
PY - 1984/11/12
Y1 - 1984/11/12
N2 - The major constituent of the trypanosomal kinetoplast ONA network are several thousand duplex DNA minicircles whose biological function is still unknown. The coding capacity and expression of these DNA minicricles, was studied in the trypanosomatid Crithidia fasciculata. Kinetoplast DNA minicircle fragments inserted into bacterial plasmid vectors were expressed in the bacterial cell. Sera elicited in rabbits, by immunization with the translational products of kinetoplast DNA minicircles in E.coli, reacted specifically with Crithidia fasciculata cellular antigens. It is inferred that kinetoplast DNA minicircles contain long open reading frames of nucleotides which are expressed in the trypanosomatid cell.
AB - The major constituent of the trypanosomal kinetoplast ONA network are several thousand duplex DNA minicircles whose biological function is still unknown. The coding capacity and expression of these DNA minicricles, was studied in the trypanosomatid Crithidia fasciculata. Kinetoplast DNA minicircle fragments inserted into bacterial plasmid vectors were expressed in the bacterial cell. Sera elicited in rabbits, by immunization with the translational products of kinetoplast DNA minicircles in E.coli, reacted specifically with Crithidia fasciculata cellular antigens. It is inferred that kinetoplast DNA minicircles contain long open reading frames of nucleotides which are expressed in the trypanosomatid cell.
UR - http://www.scopus.com/inward/record.url?scp=0021761302&partnerID=8YFLogxK
U2 - 10.1093/nar/12.21.8017
DO - 10.1093/nar/12.21.8017
M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???
C2 - 6095184
AN - SCOPUS:0021761302
SN - 0305-1048
VL - 12
SP - 8017
EP - 8028
JO - Nucleic Acids Research
JF - Nucleic Acids Research
IS - 21
ER -