Knowledge-based predictions of binding constants for engineered enzymes

Amnon Horovitz; R. D. Levine

doi:10.1016/S0022-5193(89)80236-X

Knowledge-based predictions of binding constants for engineered enzymes

Amnon Horovitz^*
, R. D. Levine

^*Corresponding author for this work

Institute of Chemistry

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Compaction of binding data for engineered enzymes is demonstrated and leads to successful knowledge-based predictions of the effects of amino-acid substitutions. Results are reported for amino-acid replacements in subtilisin from Bacillus amyloliquefaciens. The method described should contribute towards a rational design of novel protein molecules.

Original language	English
Pages (from-to)	557-562
Number of pages	6
Journal	Journal of Theoretical Biology
Volume	141
Issue number	4
DOIs	https://doi.org/10.1016/S0022-5193(89)80236-X
State	Published - 19 Dec 1989

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title = "Knowledge-based predictions of binding constants for engineered enzymes",

abstract = "Compaction of binding data for engineered enzymes is demonstrated and leads to successful knowledge-based predictions of the effects of amino-acid substitutions. Results are reported for amino-acid replacements in subtilisin from Bacillus amyloliquefaciens. The method described should contribute towards a rational design of novel protein molecules.",

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AB - Compaction of binding data for engineered enzymes is demonstrated and leads to successful knowledge-based predictions of the effects of amino-acid substitutions. Results are reported for amino-acid replacements in subtilisin from Bacillus amyloliquefaciens. The method described should contribute towards a rational design of novel protein molecules.

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Knowledge-based predictions of binding constants for engineered enzymes

Abstract

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