Label-free analysis of thrombin or Hg2+ ions by nucleic acid-functionalized graphene oxide matrices assembled on field-effect transistors

Etery Sharon, Xiaoqing Liu, Ronit Freeman, Omer Yehezkeli, Itamar Willner*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

35 Scopus citations

Abstract

Graphene oxide (GO) is deposited onto the Al2O3 gates of field-effect transistor devices to yield a functional matrix for the sensing of thrombin or Hg2+ ions. The deposition of an aptamer subunit against thrombin or a tailored nucleic acid with appropriate T mismatches on the GO, yield active surfaces for the analysis of thrombin or Hg2+ ions. The desorption of the aptamer-subunit from GO, through the formation of the aptamer-thrombin complex, or the desorption of the T-Hg2+-T-bridged duplex DNA alter the gate potential, thus providing a signal for the sensing events.

Original languageEnglish
Pages (from-to)851-856
Number of pages6
JournalElectroanalysis
Volume25
Issue number4
DOIs
StatePublished - Apr 2013

Keywords

  • Aptamer
  • DNA
  • Field-effect transistors
  • Graphene oxide
  • Sensors

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