Label-free femtomolar cancer biomarker detection in human serum using graphene-coated surface plasmon resonance chips

Lijie He, Quentin Pagneux, Iban Larroulet, Aritz Yanguas Serrano, Amaia Pesquera, Amaia Zurutuza, Daniel Mandler, Rabah Boukherroub, Sabine Szunerits*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

112 Scopus citations

Abstract

Sensitive and selective detection of cancer biomarkers is vital for the successful diagnosis of early stage cancer and follow-up treatment. Surface Plasmon Resonance (SPR) in combination with different amplification strategies is one of the analytical approaches allowing the screening of protein biomarkers in serum. Here we describe the development of a point-of-care sensor for the detection of folic acid protein (FAP) using graphene-based SPR chips. The exceptional properties of CVD graphene were exploited to construct a highly sensitive and selective SPR chip for folate biomarker sensing in serum. The specific recognition of FAP is based on the interaction between folic acid receptors integrated through π-stacking on the graphene coated SPR chip and the FAP analyte in serum. A simple post-adsorption of human serum:bovine serum albumin (HS:BSA) mixtures onto the folic acid modified sensor resulted in a highly anti-fouling interface, while keeping the sensing capabilities for folate biomarkers. This sensor allowed femtomolar (fM) detection of FAP, a detection limit well adapted and promising for quantitative clinical analysis.

Original languageAmerican English
Pages (from-to)606-611
Number of pages6
JournalBiosensors and Bioelectronics
Volume89
DOIs
StatePublished - 15 Mar 2017

Bibliographical note

Publisher Copyright:
© 2016 Elsevier B.V.

Keywords

  • Biomarker
  • Folate receptor
  • Folic acid
  • Folic acid protein
  • Graphene
  • SPR

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