Abstract
Sensitive and selective detection of cancer biomarkers is vital for the successful diagnosis of early stage cancer and follow-up treatment. Surface Plasmon Resonance (SPR) in combination with different amplification strategies is one of the analytical approaches allowing the screening of protein biomarkers in serum. Here we describe the development of a point-of-care sensor for the detection of folic acid protein (FAP) using graphene-based SPR chips. The exceptional properties of CVD graphene were exploited to construct a highly sensitive and selective SPR chip for folate biomarker sensing in serum. The specific recognition of FAP is based on the interaction between folic acid receptors integrated through π-stacking on the graphene coated SPR chip and the FAP analyte in serum. A simple post-adsorption of human serum:bovine serum albumin (HS:BSA) mixtures onto the folic acid modified sensor resulted in a highly anti-fouling interface, while keeping the sensing capabilities for folate biomarkers. This sensor allowed femtomolar (fM) detection of FAP, a detection limit well adapted and promising for quantitative clinical analysis.
| Original language | English |
|---|---|
| Pages (from-to) | 606-611 |
| Number of pages | 6 |
| Journal | Biosensors and Bioelectronics |
| Volume | 89 |
| DOIs | |
| State | Published - 15 Mar 2017 |
Bibliographical note
Publisher Copyright:© 2016 Elsevier B.V.
Keywords
- Biomarker
- Folate receptor
- Folic acid
- Folic acid protein
- Graphene
- SPR
