TY - JOUR
T1 - Large restriction fragments containing poly-TG are highly polymorphic in a variety of vertebrates
AU - Kashi, Y.
AU - Tikochinsky, Y.
AU - Genislav, E.
AU - Lraqi, F.
AU - Nave, A.
AU - Beckmann, J. S.
AU - Gruenbaum, Y.
AU - Soller, M.
PY - 1990/3/11
Y1 - 1990/3/11
N2 - Southern blots of genomic DNA from a variety of species digested by restriction endonucleases having a four-bp specificity, were probed with a bovine genomic clone consisting of seven tandem poly-TG stretches separated by a 29bp linker sequence. Highly variable DNA 'fingerprint' patterns were obtained in chicken, sheep, and horse, moderately variable DNA 'fingerprints' in mouse and man, and a monomorphic pattern in Drosophila. In chicken, horse and man a (TG)10 synthetic oligonucleotide probe gave results identical to those given by the bovine probe. Furthermore, in chicken the DNA fingerprint variation showed typical Mendelian inheritance and differed from the fingerprints obtained with Jeffreys 33.6 and M13 minisatellite probes. Thus, for a variety of vertebrate species, poty-TG-containing probes can uncover useful genetic variation.
AB - Southern blots of genomic DNA from a variety of species digested by restriction endonucleases having a four-bp specificity, were probed with a bovine genomic clone consisting of seven tandem poly-TG stretches separated by a 29bp linker sequence. Highly variable DNA 'fingerprint' patterns were obtained in chicken, sheep, and horse, moderately variable DNA 'fingerprints' in mouse and man, and a monomorphic pattern in Drosophila. In chicken, horse and man a (TG)10 synthetic oligonucleotide probe gave results identical to those given by the bovine probe. Furthermore, in chicken the DNA fingerprint variation showed typical Mendelian inheritance and differed from the fingerprints obtained with Jeffreys 33.6 and M13 minisatellite probes. Thus, for a variety of vertebrate species, poty-TG-containing probes can uncover useful genetic variation.
UR - http://www.scopus.com/inward/record.url?scp=0025219206&partnerID=8YFLogxK
U2 - 10.1093/nar/18.5.1129
DO - 10.1093/nar/18.5.1129
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C2 - 1969619
AN - SCOPUS:0025219206
SN - 0305-1048
VL - 18
SP - 1129
EP - 1132
JO - Nucleic Acids Research
JF - Nucleic Acids Research
IS - 5
ER -