Large-Scale Purification of Natural Ice-Binding Proteins by the Falling Water Ice Purification Method

Maya Bar Dolev, Chen Adar, Vera Sirotinskaya, Ido Braslavsky*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

We describe here a generic technique for purification of ice-binding proteins (IBPs) using a commercial ice machine. The method, which is called falling water ice purification (FWIP) [1], is based on the affinity of the proteins to ice, and it is therefore suitable for any IBP, natural and synthetic, with and without fused segments or domains. The FWIP method does not require the addition of tags to the protein, nor the use of resins and additives, and is suitable for large-scale purification. These features may turn FWIP useful also in the food and medical sectors.

Original languageAmerican English
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages63-71
Number of pages9
DOIs
StatePublished - 2024

Publication series

NameMethods in Molecular Biology
Volume2730
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Bibliographical note

Publisher Copyright:
© The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2024.

Keywords

  • Antifreeze protein
  • Cold finger
  • Falling water ice purification
  • Ice affinity purification
  • Ice-binding protein
  • Protein purification

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