Large-Scale Purification of Natural Ice-Binding Proteins by the Falling Water Ice Purification Method

Maya Bar Dolev; Chen Adar; Vera Sirotinskaya; Ido Braslavsky

doi:10.1007/978-1-0716-3503-2_4

Large-Scale Purification of Natural Ice-Binding Proteins by the Falling Water Ice Purification Method

Maya Bar Dolev, Chen Adar, Vera Sirotinskaya, Ido Braslavsky^*

^*Corresponding author for this work

Institute of Biochemistry, Food Science and Nutrition

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

1 Scopus citations

Abstract

We describe here a generic technique for purification of ice-binding proteins (IBPs) using a commercial ice machine. The method, which is called falling water ice purification (FWIP) [1], is based on the affinity of the proteins to ice, and it is therefore suitable for any IBP, natural and synthetic, with and without fused segments or domains. The FWIP method does not require the addition of tags to the protein, nor the use of resins and additives, and is suitable for large-scale purification. These features may turn FWIP useful also in the food and medical sectors.

Original language	English
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	63-71
Number of pages	9
DOIs	https://doi.org/10.1007/978-1-0716-3503-2_4
State	Published - 2024

Publication series

Name	Methods in Molecular Biology
Volume	2730
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Bibliographical note

Publisher Copyright:
© The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2024.

Keywords

Antifreeze protein
Cold finger
Falling water ice purification
Ice affinity purification
Ice-binding protein
Protein purification

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10.1007/978-1-0716-3503-2_4

Cite this

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Large-Scale Purification of Natural Ice-Binding Proteins by the Falling Water Ice Purification Method

Abstract

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Keywords

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