Large scale synthesis of dinucleoside monophosphates catalyzed by ribonuclease from Aspergillus clavatus

S. Bauer*, R. Lamed, Y. Lapidot

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

A gram scale enzymatic synthesis of eight, dinucleoside monophosphates (ApC, ApU, CpC, CpU, GpC, GpU, UpC, and UpU) is described. The synthesis involves a reaction between the appropriate ribonucleoside‐2′,3′‐cyclie phosphates and cytidine or uridine in the presence of ribonuelease from Aspergillus clavatus at 30°C. The enzyme is removed from the reaction mixture by chromatography on Bio‐Gel P–4, and the dinucleoside monophosphate is further purified by chromatography on a DEAE‐Sephadex A–25, column. A procedure for the large scale preparation of the ribonuclease from Aspergillus clavatus is also described.

Original languageEnglish
Pages (from-to)861-870
Number of pages10
JournalBiotechnology and Bioengineering
Volume14
Issue number6
DOIs
StatePublished - Nov 1972

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