TY - JOUR
T1 - Large scale synthesis of dinucleoside monophosphates catalyzed by ribonuclease from Aspergillus clavatus
AU - Bauer, S.
AU - Lamed, R.
AU - Lapidot, Y.
PY - 1972/11
Y1 - 1972/11
N2 - A gram scale enzymatic synthesis of eight, dinucleoside monophosphates (ApC, ApU, CpC, CpU, GpC, GpU, UpC, and UpU) is described. The synthesis involves a reaction between the appropriate ribonucleoside‐2′,3′‐cyclie phosphates and cytidine or uridine in the presence of ribonuelease from Aspergillus clavatus at 30°C. The enzyme is removed from the reaction mixture by chromatography on Bio‐Gel P–4, and the dinucleoside monophosphate is further purified by chromatography on a DEAE‐Sephadex A–25, column. A procedure for the large scale preparation of the ribonuclease from Aspergillus clavatus is also described.
AB - A gram scale enzymatic synthesis of eight, dinucleoside monophosphates (ApC, ApU, CpC, CpU, GpC, GpU, UpC, and UpU) is described. The synthesis involves a reaction between the appropriate ribonucleoside‐2′,3′‐cyclie phosphates and cytidine or uridine in the presence of ribonuelease from Aspergillus clavatus at 30°C. The enzyme is removed from the reaction mixture by chromatography on Bio‐Gel P–4, and the dinucleoside monophosphate is further purified by chromatography on a DEAE‐Sephadex A–25, column. A procedure for the large scale preparation of the ribonuclease from Aspergillus clavatus is also described.
UR - http://www.scopus.com/inward/record.url?scp=0015422501&partnerID=8YFLogxK
U2 - 10.1002/bit.260140602
DO - 10.1002/bit.260140602
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C2 - 4647693
AN - SCOPUS:0015422501
SN - 0006-3592
VL - 14
SP - 861
EP - 870
JO - Biotechnology and Bioengineering
JF - Biotechnology and Bioengineering
IS - 6
ER -