Leishmania spp.: Effect of inhibitors on growth and on polyamine and macromolecular syntheses

U. Bachrach; S. Brem; S. B. Wertman; L. F. Schnur; C. L. Greenblatt

doi:10.1016/0014-4894(79)90131-0

Leishmania spp. Effect of inhibitors on growth and on polyamine and macromolecular syntheses

U. Bachrach^*, S. Brem, S. B. Wertman, L. F. Schnur, C. L. Greenblatt

^*Corresponding author for this work

Institute for Medical Research Israel-Canada (IMRIC)

Research output: Contribution to journal › Article › peer-review

34 Scopus citations

Abstract

Ethidium bromide, pentamidine isethionate, and MGBG [methylglyoxal-bis (guanylhydrazone)] inhibited the uptake of radioactive putrescine by leishmanial (Leishmania spp.; Leishmania tropica major; Leishmania mexicana; Leishmania donovani) promastigotes and interfered with their polyamine synthesis. Inhibition was apparent as early as 1 hr after adding these drugs to the parasites at growth-inhibiting concentrations. Ethidium bromide also inhibited the incorporation of radioactive uracil into leishmanial RNA at growth-inhibiting concentrations, while DNA synthesis was inhibited by ethidium bromide at high concentrations after a lag period. MGBG inhibited the synthesis of leishmanial DNA and RNA at growth-inhibiting concentrations.

Original language	English
Pages (from-to)	464-470
Number of pages	7
Journal	Experimental Parasitology
Volume	48
Issue number	3
DOIs	https://doi.org/10.1016/0014-4894(79)90131-0
State	Published - Dec 1979

Keywords

Culture
Ethidium bromide
Growth cycle
Hemoflagellates
Inhibitors
Leishmania donovani Protozoa, parasitic
Leishmania mexicana
Leishmania spp.
Leishmania tropica major
MGBG [methylglyoxal-bis (guanylhydrazone)
Pentamidine
Polyamines
Putrescine
Spermidine
Spermine

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10.1016/0014-4894(79)90131-0

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title = "Leishmania spp.: Effect of inhibitors on growth and on polyamine and macromolecular syntheses",

abstract = "Ethidium bromide, pentamidine isethionate, and MGBG [methylglyoxal-bis (guanylhydrazone)] inhibited the uptake of radioactive putrescine by leishmanial (Leishmania spp.; Leishmania tropica major; Leishmania mexicana; Leishmania donovani) promastigotes and interfered with their polyamine synthesis. Inhibition was apparent as early as 1 hr after adding these drugs to the parasites at growth-inhibiting concentrations. Ethidium bromide also inhibited the incorporation of radioactive uracil into leishmanial RNA at growth-inhibiting concentrations, while DNA synthesis was inhibited by ethidium bromide at high concentrations after a lag period. MGBG inhibited the synthesis of leishmanial DNA and RNA at growth-inhibiting concentrations.",

keywords = "Culture, Ethidium bromide, Growth cycle, Hemoflagellates, Inhibitors, Leishmania donovani Protozoa, parasitic, Leishmania mexicana, Leishmania spp., Leishmania tropica major, MGBG [methylglyoxal-bis (guanylhydrazone), Pentamidine, Polyamines, Putrescine, Spermidine, Spermine",

author = "U. Bachrach and S. Brem and Wertman, {S. B.} and Schnur, {L. F.} and Greenblatt, {C. L.}",

year = "1979",

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AU - Schnur, L. F.

AU - Greenblatt, C. L.

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N2 - Ethidium bromide, pentamidine isethionate, and MGBG [methylglyoxal-bis (guanylhydrazone)] inhibited the uptake of radioactive putrescine by leishmanial (Leishmania spp.; Leishmania tropica major; Leishmania mexicana; Leishmania donovani) promastigotes and interfered with their polyamine synthesis. Inhibition was apparent as early as 1 hr after adding these drugs to the parasites at growth-inhibiting concentrations. Ethidium bromide also inhibited the incorporation of radioactive uracil into leishmanial RNA at growth-inhibiting concentrations, while DNA synthesis was inhibited by ethidium bromide at high concentrations after a lag period. MGBG inhibited the synthesis of leishmanial DNA and RNA at growth-inhibiting concentrations.

AB - Ethidium bromide, pentamidine isethionate, and MGBG [methylglyoxal-bis (guanylhydrazone)] inhibited the uptake of radioactive putrescine by leishmanial (Leishmania spp.; Leishmania tropica major; Leishmania mexicana; Leishmania donovani) promastigotes and interfered with their polyamine synthesis. Inhibition was apparent as early as 1 hr after adding these drugs to the parasites at growth-inhibiting concentrations. Ethidium bromide also inhibited the incorporation of radioactive uracil into leishmanial RNA at growth-inhibiting concentrations, while DNA synthesis was inhibited by ethidium bromide at high concentrations after a lag period. MGBG inhibited the synthesis of leishmanial DNA and RNA at growth-inhibiting concentrations.

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KW - Ethidium bromide

KW - Growth cycle

KW - Hemoflagellates

KW - Inhibitors

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KW - Leishmania mexicana

KW - Leishmania spp.

KW - Leishmania tropica major

KW - MGBG [methylglyoxal-bis (guanylhydrazone)

KW - Pentamidine

KW - Polyamines

KW - Putrescine

KW - Spermidine

KW - Spermine

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Leishmania spp. Effect of inhibitors on growth and on polyamine and macromolecular syntheses

Abstract

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