TY - JOUR
T1 - Leishmania tropica and Leishmania donovani
T2 - Solid phase radioimmunoassay using leishmanial excreted factor
AU - El-On, Joseph
AU - Zehavi, Uri
AU - Avraham, Hava
AU - Greenblatt, Charles L.
PY - 1983/6
Y1 - 1983/6
N2 - A radioimmunoassay for the quantitative determination of anti-leishmanial excreted factor (EF) antibody in rabbit sera was developed. The assay, using Leishmania tropica and Leishmania donovani promastigotes EF, purified by either extraction with phenol followed by fractionation on a Sephadex G-100 column or by the dissociation of EF antibody complexes, was shown to be sensitive and reproducible. Using monospecific anti-EF antibodies, levels of as low as 0.06-0.12 μg/ml of anti-EF IgG could be detected. The specificity of the assay was assessed by inhibition with homologous and heterologous EF. Only minor cross-reactivity with heterologous EF was observed, and as little as 2.5 μg/ml of EF could be detected. Sera from kala-azar patients showed only 1.8-3.1 times more anti-EF activity, as compared with uninfected controls. No specificity was observed with sera from kala-azar patients with regard to the type of EF used. Almost the same activity was obtained with both EF from L. tropica and L. donovani. No anti-EF antibodies were detected in sera from patients with cutaneous leishmaniasis.
AB - A radioimmunoassay for the quantitative determination of anti-leishmanial excreted factor (EF) antibody in rabbit sera was developed. The assay, using Leishmania tropica and Leishmania donovani promastigotes EF, purified by either extraction with phenol followed by fractionation on a Sephadex G-100 column or by the dissociation of EF antibody complexes, was shown to be sensitive and reproducible. Using monospecific anti-EF antibodies, levels of as low as 0.06-0.12 μg/ml of anti-EF IgG could be detected. The specificity of the assay was assessed by inhibition with homologous and heterologous EF. Only minor cross-reactivity with heterologous EF was observed, and as little as 2.5 μg/ml of EF could be detected. Sera from kala-azar patients showed only 1.8-3.1 times more anti-EF activity, as compared with uninfected controls. No specificity was observed with sera from kala-azar patients with regard to the type of EF used. Almost the same activity was obtained with both EF from L. tropica and L. donovani. No anti-EF antibodies were detected in sera from patients with cutaneous leishmaniasis.
KW - Excreted factor, EF
KW - Kala-azar
KW - Leishmania donovani
KW - Leishmania tropica
KW - Leishmaniasis, cutaneous
KW - Protozoa, parasitic
KW - Radioimmunoassay
UR - http://www.scopus.com/inward/record.url?scp=0020967778&partnerID=8YFLogxK
U2 - 10.1016/0014-4894(83)90023-1
DO - 10.1016/0014-4894(83)90023-1
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C2 - 6852165
AN - SCOPUS:0020967778
SN - 0014-4894
VL - 55
SP - 270
EP - 279
JO - Experimental Parasitology
JF - Experimental Parasitology
IS - 3
ER -