Leishmania tropica: Intraspecific polymorphisms in lipophosphoglycan correlate with transmission by different Phlebotomus species

Rodrigo P.P. Soares; Tamara Barron; Kessler McCoy-Simandle; Milena Svobodova; Alon Warburg; Salvatore J. Turco

doi:10.1016/j.exppara.2004.05.001

Leishmania tropica: Intraspecific polymorphisms in lipophosphoglycan correlate with transmission by different Phlebotomus species

Rodrigo P.P. Soares, Tamara Barron, Kessler McCoy-Simandle, Milena Svobodova, Alon Warburg, Salvatore J. Turco^*

^*Corresponding author for this work

Department of Microbiology and Molecular Genetics

Research output: Contribution to journal › Article › peer-review

38 Scopus citations

Abstract

Lipophosphoglycan (LPG) is a dominant surface molecule of Leishmania promastigotes which has been shown to be critical for parasite-sand fly vector interactions. To provide additional evidence for its importance in transmission, the LPGs from three Leishmania tropica strains that differ in their capability to infect sand flies, were biochemically characterized. One of these strains, ISER/IL/98/LRC-L747, was isolated from a Phlebotomus sergenti female collected in the Judean Desert close to Jerusalem. The other strains originated from a different focus in the Galilee region of northern Israel. One was isolated from a patient (MHOM/IL/02/Ofri-LRC-L863) and the other from a naturally infected Phlebotomus arabicus female (IARA/IL/00/Amnunfly1-LRC-L810). The LPG structures of the isolates from the Galilee (L863 and L810) were similar to each other, but differed in the LPG repeat units from the Judean Desert isolate (L747). The terminal sugar in the side chains of the repeat units of LPG purified from L863 and L810 was β-galactose and was not capped with glucose, unlike L747 and a previously characterized L. tropica strain from Iraq (L36). Since L810 was isolated from P. arabicus and L747 from P. sergenti, variations in the structure of their LPGs may explain their capacity to infect different sand fly species. Index Descriptors and Abbreviations: LPG, lipophosphoglycan; PBS, phosphate-buffered saline; FACE, fluorophore-assisted carbohydrate electrophoresis; CE, capillary electrophoresis, ANTS; 8-aminonaphthalene-1,3,6- trisulfate; APTS, 8-aminopyrene-1,3,6-trisulfonic acid trisodium salt

Original language	English
Pages (from-to)	105-114
Number of pages	10
Journal	Experimental Parasitology
Volume	107
Issue number	1-2
DOIs	https://doi.org/10.1016/j.exppara.2004.05.001
State	Published - May 2004

Bibliographical note

Funding Information:
M. Svobodova is supported by Grant Agency of the Czech Republic, 206/02/P107. S.J. Turco is supported by National Institutes of Health Grant AI20941. A. Warburg was supported by Grant number SO 220/5-1 from the Deutsche Forschungsgemeinschaft (DFG): “The German–Israeli–Palestinian Cooperative project on Leishmaniasis in Israel and The West Bank.”

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@article{c69e88d8f717459e996030b69dd95c7b,

title = "Leishmania tropica: Intraspecific polymorphisms in lipophosphoglycan correlate with transmission by different Phlebotomus species",

abstract = "Lipophosphoglycan (LPG) is a dominant surface molecule of Leishmania promastigotes which has been shown to be critical for parasite-sand fly vector interactions. To provide additional evidence for its importance in transmission, the LPGs from three Leishmania tropica strains that differ in their capability to infect sand flies, were biochemically characterized. One of these strains, ISER/IL/98/LRC-L747, was isolated from a Phlebotomus sergenti female collected in the Judean Desert close to Jerusalem. The other strains originated from a different focus in the Galilee region of northern Israel. One was isolated from a patient (MHOM/IL/02/Ofri-LRC-L863) and the other from a naturally infected Phlebotomus arabicus female (IARA/IL/00/Amnunfly1-LRC-L810). The LPG structures of the isolates from the Galilee (L863 and L810) were similar to each other, but differed in the LPG repeat units from the Judean Desert isolate (L747). The terminal sugar in the side chains of the repeat units of LPG purified from L863 and L810 was β-galactose and was not capped with glucose, unlike L747 and a previously characterized L. tropica strain from Iraq (L36). Since L810 was isolated from P. arabicus and L747 from P. sergenti, variations in the structure of their LPGs may explain their capacity to infect different sand fly species. Index Descriptors and Abbreviations: LPG, lipophosphoglycan; PBS, phosphate-buffered saline; FACE, fluorophore-assisted carbohydrate electrophoresis; CE, capillary electrophoresis, ANTS; 8-aminonaphthalene-1,3,6- trisulfate; APTS, 8-aminopyrene-1,3,6-trisulfonic acid trisodium salt",

author = "Soares, {Rodrigo P.P.} and Tamara Barron and Kessler McCoy-Simandle and Milena Svobodova and Alon Warburg and Turco, {Salvatore J.}",

note = "Funding Information: M. Svobodova is supported by Grant Agency of the Czech Republic, 206/02/P107. S.J. Turco is supported by National Institutes of Health Grant AI20941. A. Warburg was supported by Grant number SO 220/5-1 from the Deutsche Forschungsgemeinschaft (DFG): “The German–Israeli–Palestinian Cooperative project on Leishmaniasis in Israel and The West Bank.”",

year = "2004",

month = may,

doi = "10.1016/j.exppara.2004.05.001",

language = "אנגלית",

volume = "107",

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AU - McCoy-Simandle, Kessler

AU - Svobodova, Milena

AU - Warburg, Alon

AU - Turco, Salvatore J.

N1 - Funding Information: M. Svobodova is supported by Grant Agency of the Czech Republic, 206/02/P107. S.J. Turco is supported by National Institutes of Health Grant AI20941. A. Warburg was supported by Grant number SO 220/5-1 from the Deutsche Forschungsgemeinschaft (DFG): “The German–Israeli–Palestinian Cooperative project on Leishmaniasis in Israel and The West Bank.”

PY - 2004/5

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N2 - Lipophosphoglycan (LPG) is a dominant surface molecule of Leishmania promastigotes which has been shown to be critical for parasite-sand fly vector interactions. To provide additional evidence for its importance in transmission, the LPGs from three Leishmania tropica strains that differ in their capability to infect sand flies, were biochemically characterized. One of these strains, ISER/IL/98/LRC-L747, was isolated from a Phlebotomus sergenti female collected in the Judean Desert close to Jerusalem. The other strains originated from a different focus in the Galilee region of northern Israel. One was isolated from a patient (MHOM/IL/02/Ofri-LRC-L863) and the other from a naturally infected Phlebotomus arabicus female (IARA/IL/00/Amnunfly1-LRC-L810). The LPG structures of the isolates from the Galilee (L863 and L810) were similar to each other, but differed in the LPG repeat units from the Judean Desert isolate (L747). The terminal sugar in the side chains of the repeat units of LPG purified from L863 and L810 was β-galactose and was not capped with glucose, unlike L747 and a previously characterized L. tropica strain from Iraq (L36). Since L810 was isolated from P. arabicus and L747 from P. sergenti, variations in the structure of their LPGs may explain their capacity to infect different sand fly species. Index Descriptors and Abbreviations: LPG, lipophosphoglycan; PBS, phosphate-buffered saline; FACE, fluorophore-assisted carbohydrate electrophoresis; CE, capillary electrophoresis, ANTS; 8-aminonaphthalene-1,3,6- trisulfate; APTS, 8-aminopyrene-1,3,6-trisulfonic acid trisodium salt

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Leishmania tropica: Intraspecific polymorphisms in lipophosphoglycan correlate with transmission by different Phlebotomus species

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