TY - JOUR
T1 - Leishmanial excreted factors (EFs)
T2 - Purification by affinity chromatography
AU - Zehavi, Uri
AU - Abrahams, Judith C.
AU - Granoth, Ruth
AU - Greenblatt, Charles L.
AU - Slutzky, Gerald M.
AU - El-On, Joseph
PY - 1983/11
Y1 - 1983/11
N2 - Leishmania species grown in culture excrete a polyanionic, carbohydrate-rich factor (EF) which binds to antibodies produced in rabbits against the parent Leishmania species. EF, previously purified by physical and chemical methods, was purified by affinity chromatography on a Ricinus lectin column. The purified samples were characterised and analysed. The results show a notable proportion of galactose in EF and clarify the reasons for its polyanionic properties. Heterogenicity of EF is demonstrated and discussed.
AB - Leishmania species grown in culture excrete a polyanionic, carbohydrate-rich factor (EF) which binds to antibodies produced in rabbits against the parent Leishmania species. EF, previously purified by physical and chemical methods, was purified by affinity chromatography on a Ricinus lectin column. The purified samples were characterised and analysed. The results show a notable proportion of galactose in EF and clarify the reasons for its polyanionic properties. Heterogenicity of EF is demonstrated and discussed.
UR - http://www.scopus.com/inward/record.url?scp=0021080828&partnerID=8YFLogxK
U2 - 10.1007/BF00927419
DO - 10.1007/BF00927419
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C2 - 6659650
AN - SCOPUS:0021080828
SN - 0044-3255
VL - 69
SP - 695
EP - 701
JO - Parasitology Research
JF - Parasitology Research
IS - 6
ER -