Leptin modulates lymphocytes' adherence to hepatic stellate cells is associated with oxidative status alterations

Lina Abu-Tair; Sarit Doron; Mahmud Mahamid; Johnny Amer; Rifaat Safadi

doi:10.1016/j.mito.2012.10.012

Leptin modulates lymphocytes' adherence to hepatic stellate cells is associated with oxidative status alterations

Lina Abu-Tair
, Sarit Doron
, Mahmud Mahamid
, Johnny Amer^*
, Rifaat Safadi

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

We investigated leptin effects on lymphocyte interactions with hepatic-stellate-cells (HSCs). Leptin showed pro-fibrotic effects on HSCs with oxidative status imbalance.In co-cultures, leptin activates HSCs and consequently adhered HCV-lymphocytes more than healthy ones. Leptin also increased healthy and HCV lymphocyte proliferations; increased their reactive-oxygen-species; decreased antioxidants (reduced-glutathione) levels while inhibited apoptosis only of HCV-lymphocytes. The leptin-treated HCV-lymphocytes activated HSCs, increase interleukin-4 while decreased their apoptosis.Leptin-receptor-deficient (db-. db)-HSCs did not adhere lymphocytes. db/. db-lymphocytes however showed fewer adherences to HSCs when compared to WT-counterparts.This study presents immune and oxidative modulatory effects of leptin on lymphocytes and their consequent interaction with HSCs.

Original language	English
Pages (from-to)	473-480
Number of pages	8
Journal	Mitochondrion
Volume	13
Issue number	5
DOIs	https://doi.org/10.1016/j.mito.2012.10.012
State	Published - Sep 2013
Externally published	Yes

Keywords

Adherence
Hepatic fibrosis
Leptin receptor
Lymphocytes
Oxidative stress

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10.1016/j.mito.2012.10.012

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title = "Leptin modulates lymphocytes' adherence to hepatic stellate cells is associated with oxidative status alterations",

abstract = "We investigated leptin effects on lymphocyte interactions with hepatic-stellate-cells (HSCs). Leptin showed pro-fibrotic effects on HSCs with oxidative status imbalance.In co-cultures, leptin activates HSCs and consequently adhered HCV-lymphocytes more than healthy ones. Leptin also increased healthy and HCV lymphocyte proliferations; increased their reactive-oxygen-species; decreased antioxidants (reduced-glutathione) levels while inhibited apoptosis only of HCV-lymphocytes. The leptin-treated HCV-lymphocytes activated HSCs, increase interleukin-4 while decreased their apoptosis.Leptin-receptor-deficient (db-. db)-HSCs did not adhere lymphocytes. db/. db-lymphocytes however showed fewer adherences to HSCs when compared to WT-counterparts.This study presents immune and oxidative modulatory effects of leptin on lymphocytes and their consequent interaction with HSCs.",

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author = "Lina Abu-Tair and Sarit Doron and Mahmud Mahamid and Johnny Amer and Rifaat Safadi",

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AU - Abu-Tair, Lina

AU - Doron, Sarit

AU - Mahamid, Mahmud

AU - Amer, Johnny

AU - Safadi, Rifaat

PY - 2013/9

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AB - We investigated leptin effects on lymphocyte interactions with hepatic-stellate-cells (HSCs). Leptin showed pro-fibrotic effects on HSCs with oxidative status imbalance.In co-cultures, leptin activates HSCs and consequently adhered HCV-lymphocytes more than healthy ones. Leptin also increased healthy and HCV lymphocyte proliferations; increased their reactive-oxygen-species; decreased antioxidants (reduced-glutathione) levels while inhibited apoptosis only of HCV-lymphocytes. The leptin-treated HCV-lymphocytes activated HSCs, increase interleukin-4 while decreased their apoptosis.Leptin-receptor-deficient (db-. db)-HSCs did not adhere lymphocytes. db/. db-lymphocytes however showed fewer adherences to HSCs when compared to WT-counterparts.This study presents immune and oxidative modulatory effects of leptin on lymphocytes and their consequent interaction with HSCs.

KW - Adherence

KW - Hepatic fibrosis

KW - Leptin receptor

KW - Lymphocytes

KW - Oxidative stress

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IS - 5

ER -

Leptin modulates lymphocytes' adherence to hepatic stellate cells is associated with oxidative status alterations

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