Lethal bacterial trap: Cationic surface for endodontic sealing

Dana Kesler Shvero, Nathan Zaltsman, Ervin I. Weiss, David Polak, Ronen Hazan, Nurit Beyth*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

Insoluble antibacterial cationic nanoparticles have been previously shown to have potent and long-lasting antibacterial properties. Our tested hypothesis was that root canal pathogens will be attracted to and eliminated when exposed to epoxy resin-based surfaces incorporating cationic nanoparticles. In our research, an epoxy resin-based surface incorporating quaternary ammonium polyethyleneimine (QPEI) nanoparticles was evaluated. Surface characterization was performed using atomic force microscopy and X-ray photoelectron spectra. The surface anti-Enterococcus faecalis effect was evaluated in an anti-gravitational model. Cell membrane potential, viability, biofilm thickness, and biomass were tested using flow cytometry and confocal laser scanning microscopy. Additionally, the antibiofilm activity of the bacterial supernatant was assessed. The surface characterization showed QPEI nanoparticle embedment on the modified sealer. The epoxy resin-based surface incorporating the QPEI nanoparticles actively attracted bacteria, causing membrane destabilization, and bacterial death. The supernatant of bacteria pre-exposed to QPEI showed an antibacterial effect. In conclusion, the tested epoxy resin-based surface incorporating QPEI nanoparticles traps and kills bacteria. The nanoparticles attracted bacteria, reducing their viability, and promoting cell death.

Original languageAmerican English
Pages (from-to)427-434
Number of pages8
JournalJournal of Biomedical Materials Research - Part A
Volume104
Issue number2
DOIs
StatePublished - 1 Feb 2016
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2015 Wiley Periodicals, Inc.

Keywords

  • Enterococcus faecalis
  • antibacterial
  • membrane
  • nanoparticles
  • quaternary ammonium

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