TY - JOUR
T1 - Leukocyte CD300a Contributes to the Resolution of Murine Allergic Inflammation
AU - Karra, Laila
AU - Gangwar, Roopesh Singh
AU - Shamri, Revital
AU - Puzzovio, Pier Giorgio
AU - Cohen-Mor, Shahar
AU - Levy, Bruce D.
AU - Levi-Schaffer, Francesca
N1 - Publisher Copyright:
Copyright © 2018 by The American Association of Immunologists, Inc. All rights reserved
PY - 2018/11/15
Y1 - 2018/11/15
N2 - CD300a is an inhibitory receptor for mast cells and eosinophils in allergic inflammation (AI); however, the spatiotemporal expression of CD300a and its potential roles in the resolution of AI are still to be determined. In this study, employing a mouse model of allergic peritonitis, we demonstrate that CD300a expression on peritoneal cells is regulated from inflammation to resolution. Allergic peritonitis-induced CD300a2/2 mice had a rapid increase in their inflammatory cell infiltrates and tryptase content in the peritoneal cavity compared with wild type, and their resolution process was significantly delayed. CD300a2/2 mice expressed lower levels of ALX/FPR2 receptor on peritoneal cells and had higher levels of LXA4 in the peritoneal lavage. CD300a activation on mouse bone marrow-derived mast cells regulated ALX/FPR2 expression levels following IgE-mediated activation. Together, these findings indicate a role for CD300a in AI and its resolution, in part via the specialized proresolving mediator LXA4 and ALX/FPR2 receptor pathway activation. The Journal of Immunology, 2018, 201: 2998-3005.
AB - CD300a is an inhibitory receptor for mast cells and eosinophils in allergic inflammation (AI); however, the spatiotemporal expression of CD300a and its potential roles in the resolution of AI are still to be determined. In this study, employing a mouse model of allergic peritonitis, we demonstrate that CD300a expression on peritoneal cells is regulated from inflammation to resolution. Allergic peritonitis-induced CD300a2/2 mice had a rapid increase in their inflammatory cell infiltrates and tryptase content in the peritoneal cavity compared with wild type, and their resolution process was significantly delayed. CD300a2/2 mice expressed lower levels of ALX/FPR2 receptor on peritoneal cells and had higher levels of LXA4 in the peritoneal lavage. CD300a activation on mouse bone marrow-derived mast cells regulated ALX/FPR2 expression levels following IgE-mediated activation. Together, these findings indicate a role for CD300a in AI and its resolution, in part via the specialized proresolving mediator LXA4 and ALX/FPR2 receptor pathway activation. The Journal of Immunology, 2018, 201: 2998-3005.
UR - http://www.scopus.com/inward/record.url?scp=85056277412&partnerID=8YFLogxK
U2 - 10.4049/jimmunol.1801000
DO - 10.4049/jimmunol.1801000
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C2 - 30315138
AN - SCOPUS:85056277412
SN - 0022-1767
VL - 201
SP - 2998
EP - 3005
JO - Journal of Immunology
JF - Journal of Immunology
IS - 10
ER -