TY - JOUR
T1 - Level of heterozygosity and mode of inheritance of variable number of tandem repeat loci in avocado
AU - Mhameed, Samir
AU - Sharon, Dror
AU - Hillel, Jossi
AU - Lahav, Emanuel
AU - Kaufman, Daniel
AU - Lavi, Uri
PY - 1996/9
Y1 - 1996/9
N2 - To estimate heterozygosity level in the avocado (Persea americana Mill.) genome, two types of variable number of tandem repeat (VNTR) markers were used. Multilocus DNA fingerprints (DFPs) were analyzed on avocado progeny resulting from either crosses or selfing of cultivars. In five crosses, heterozygosity was 100%, while in two self-pollinated families, heterozygosity was 90% and 94%. Single locus, simple sequence repeat (SSR) DNA markers were analyzed by typing 59 loci on five avocado cultivars. Average heterozygosity varied from 11.50 to 0.66, while gene diversity varied from 11.42 to 0.66. Heterozygosity varied from 38% to 70%. The percentage of fragments that exhibited Mendelian inheritance was 62.5% to 85% (P < 0.05) for the DFP fragments and 85% for the SSR alleles.
AB - To estimate heterozygosity level in the avocado (Persea americana Mill.) genome, two types of variable number of tandem repeat (VNTR) markers were used. Multilocus DNA fingerprints (DFPs) were analyzed on avocado progeny resulting from either crosses or selfing of cultivars. In five crosses, heterozygosity was 100%, while in two self-pollinated families, heterozygosity was 90% and 94%. Single locus, simple sequence repeat (SSR) DNA markers were analyzed by typing 59 loci on five avocado cultivars. Average heterozygosity varied from 11.50 to 0.66, while gene diversity varied from 11.42 to 0.66. Heterozygosity varied from 38% to 70%. The percentage of fragments that exhibited Mendelian inheritance was 62.5% to 85% (P < 0.05) for the DFP fragments and 85% for the SSR alleles.
KW - DNA fingerprint
KW - VNTR markers
KW - breeding
KW - simple sequence repeat
UR - http://www.scopus.com/inward/record.url?scp=0030473086&partnerID=8YFLogxK
U2 - 10.21273/jashs.121.5.768
DO - 10.21273/jashs.121.5.768
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AN - SCOPUS:0030473086
SN - 0003-1062
VL - 121
SP - 768
EP - 772
JO - Journal of the American Society for Horticultural Science
JF - Journal of the American Society for Horticultural Science
IS - 5
ER -