TY - JOUR
T1 - Light-dependent proton and rubidium translocation in membrane vesicles from Halobacterium halobium
AU - Kanner, Baruch I.
AU - Racker, Efraim
PY - 1975/6/2
Y1 - 1975/6/2
N2 - A procedure for the isolation of membrane vesicles after sonication of Halobacterium halobium is described. Upon illumination these vesicles took up rubidium. This process was stimulated 3 to 7 fold by valinomycin, and inhibited by uncouplers of oxidative phosphorylation or by nigericin. In the light, these vesicles extruded protons. However, on addition of low concentrations of uncoupler the direction of proton movement was reversed. All proton movements were abolished by high concentrations of uncoupler or by nigericin. These observations suggest that part of the vesicle population was inverted and less sensitive to uncouplers.
AB - A procedure for the isolation of membrane vesicles after sonication of Halobacterium halobium is described. Upon illumination these vesicles took up rubidium. This process was stimulated 3 to 7 fold by valinomycin, and inhibited by uncouplers of oxidative phosphorylation or by nigericin. In the light, these vesicles extruded protons. However, on addition of low concentrations of uncoupler the direction of proton movement was reversed. All proton movements were abolished by high concentrations of uncoupler or by nigericin. These observations suggest that part of the vesicle population was inverted and less sensitive to uncouplers.
UR - http://www.scopus.com/inward/record.url?scp=0016721617&partnerID=8YFLogxK
U2 - 10.1016/0006-291X(75)90154-0
DO - 10.1016/0006-291X(75)90154-0
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C2 - 238520
AN - SCOPUS:0016721617
SN - 0006-291X
VL - 64
SP - 1054
EP - 1061
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -