Limits to Resolution of CW STED Microscopy

Alexei S. Trifonov; Jean Christophe Jaskula; Claire Teulon; David R. Glenn; Nir Bar-Gill; Ronald L. Walsworth

doi:10.1016/B978-0-12-408090-4.00005-0

Limits to Resolution of CW STED Microscopy

Alexei S. Trifonov^*, Jean Christophe Jaskula, Claire Teulon, David R. Glenn, Nir Bar-Gill, Ronald L. Walsworth

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

6 Scopus citations

Abstract

We report a systematic theoretical and experimental study of the limits to spatial resolution for stimulated emission depletion (STED) superresolution fluorescence microscopy using continuous wave (CW) laser beams. We develop a theoretical framework for CW STED imaging from point fluorescent emitters and calculate the dependence of 2D spatial resolution on the power of the CW excitation (pump) beam, as well as the power, contrast, and polarization of the CW STED "doughnut" beam. We perform CW STED experiments on (non-bleaching) nitrogen vacancy (NV) color centers in diamond and find good agreement with the theoretical expressions for CW STED spatial resolution. Our results will aid the optimization and application of CW STED microscopy in both the physical and life sciences.

Original language	English
Title of host publication	Advances in Atomic, Molecular and Optical Physics
Publisher	Academic Press Inc.
Pages	279-302
Number of pages	24
DOIs	https://doi.org/10.1016/B978-0-12-408090-4.00005-0
State	Published - 2013
Externally published	Yes

Publication series

Name	Advances in Atomic, Molecular and Optical Physics
Volume	62
ISSN (Print)	1049-250X

Bibliographical note

Funding Information:
We gratefully acknowledge helpful discussions with Huiliang Zhang, David Le Sage, Chinmay Belthangady, Linh Pham, Peter Maurer, Silvia Arroyo Camejo, and Stefan Hell. This work was supported by the DARPA QuASAR program and by the NSF.

Keywords

Optical microscopy
Superresolution

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10.1016/B978-0-12-408090-4.00005-0

Cite this

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title = "Limits to Resolution of CW STED Microscopy",

abstract = "We report a systematic theoretical and experimental study of the limits to spatial resolution for stimulated emission depletion (STED) superresolution fluorescence microscopy using continuous wave (CW) laser beams. We develop a theoretical framework for CW STED imaging from point fluorescent emitters and calculate the dependence of 2D spatial resolution on the power of the CW excitation (pump) beam, as well as the power, contrast, and polarization of the CW STED {"}doughnut{"} beam. We perform CW STED experiments on (non-bleaching) nitrogen vacancy (NV) color centers in diamond and find good agreement with the theoretical expressions for CW STED spatial resolution. Our results will aid the optimization and application of CW STED microscopy in both the physical and life sciences.",

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note = "Funding Information: We gratefully acknowledge helpful discussions with Huiliang Zhang, David Le Sage, Chinmay Belthangady, Linh Pham, Peter Maurer, Silvia Arroyo Camejo, and Stefan Hell. This work was supported by the DARPA QuASAR program and by the NSF. ",

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AU - Trifonov, Alexei S.

AU - Jaskula, Jean Christophe

AU - Teulon, Claire

AU - Glenn, David R.

AU - Bar-Gill, Nir

AU - Walsworth, Ronald L.

N1 - Funding Information: We gratefully acknowledge helpful discussions with Huiliang Zhang, David Le Sage, Chinmay Belthangady, Linh Pham, Peter Maurer, Silvia Arroyo Camejo, and Stefan Hell. This work was supported by the DARPA QuASAR program and by the NSF.

PY - 2013

Y1 - 2013

N2 - We report a systematic theoretical and experimental study of the limits to spatial resolution for stimulated emission depletion (STED) superresolution fluorescence microscopy using continuous wave (CW) laser beams. We develop a theoretical framework for CW STED imaging from point fluorescent emitters and calculate the dependence of 2D spatial resolution on the power of the CW excitation (pump) beam, as well as the power, contrast, and polarization of the CW STED "doughnut" beam. We perform CW STED experiments on (non-bleaching) nitrogen vacancy (NV) color centers in diamond and find good agreement with the theoretical expressions for CW STED spatial resolution. Our results will aid the optimization and application of CW STED microscopy in both the physical and life sciences.

AB - We report a systematic theoretical and experimental study of the limits to spatial resolution for stimulated emission depletion (STED) superresolution fluorescence microscopy using continuous wave (CW) laser beams. We develop a theoretical framework for CW STED imaging from point fluorescent emitters and calculate the dependence of 2D spatial resolution on the power of the CW excitation (pump) beam, as well as the power, contrast, and polarization of the CW STED "doughnut" beam. We perform CW STED experiments on (non-bleaching) nitrogen vacancy (NV) color centers in diamond and find good agreement with the theoretical expressions for CW STED spatial resolution. Our results will aid the optimization and application of CW STED microscopy in both the physical and life sciences.

KW - Optical microscopy

KW - Superresolution

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T3 - Advances in Atomic, Molecular and Optical Physics

SP - 279

EP - 302

BT - Advances in Atomic, Molecular and Optical Physics

PB - Academic Press Inc.

ER -

Limits to Resolution of CW STED Microscopy

Abstract

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Bibliographical note

Keywords

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