Linker-based GnRHh-PE chimeric proteins inhibit cancer growth in nude mice

Ahmi Ben-Yehudah, Shai Yarkoni, Amotz Nechushtan, Ruth Belostotsky, Haya Lorberboum-Galski*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

Since the number of cancer-related deaths has not decreased in recent years, major efforts are being made to find new drugs for cancer treatment. In this report we introduce the gonadotropin releasing hormone-Pseudomonas exotoxin (GnRH-PE) based chimeric proteins L-GnRH-PE66 and L-GnRH-PE40. These proteins are composed of a GnRH moiety attached to modified forms of Pseudomonas exotoxin via a polylinker (gly4ser)2. The chimeric proteins L- GnRH-PE66 and L-GnRH-PE40 have the ability to target and kill adenocarcinoma cell lines in vitro, whereas non-adenocarcinoma cell lines are not affected. We demonstrate that L-GnRH-PE66 and L-GnRH-PE40 efficiently inhibit cancer growth. Nude mice were injected subcutaneously with the SW-48 adenocarcinoma cell line to produce xenograft tumours. When the tumours were established and visible, the animals were injected with chimeric proteins for 10 days. At the end of this period, a reduction of up to 3-fold in tumor size was obtained in the treated mice, as compared with the control group, which received equivalent amounts of GnRH; the difference was even greater 13 days after termination of treatment. Thus, the chimeric proteins L-GnRH-PE66 and L- GnRH-PE40 are promising candidates for treatment of a variety of adenocarcinomas and their use in humans should be considered.

Original languageAmerican English
Pages (from-to)38-45
Number of pages8
JournalMedical Oncology
Volume16
Issue number1
StatePublished - Apr 1999

Keywords

  • Cancer
  • Chimeric proteins
  • Gonadotropin releasing hormone (GnRH)
  • Pseudomonas exotoxin A (PE)
  • Targeting

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