The immunomodulatory potential of a diet enriched with n-3 polyunsaturated fatty acids was analysed in naive mice with experimental antiphospholipid syndrome (APS) induced by active immunization with H-3, a human anti-beta-2-glycoprotein-I (anti-βGPI) monoclonal antibody (mAb). Fetal loss and other clinical manifestations of APS were prevented in the group of APS mice upon exposure to the enriched n-3 diet compared to the control group. The titers of anti-βGPI were significantly lower (in O.D. at 405 nm, 1.387±0.232 in comparison to non-treated mice 0.637±0.111, P<0.05). The reduced titer of anti-βGPI antibodies in the sera of the treated mice was associated with a reduced number of anti-βGPI forming cells in cultured splenocytes (84±14, antibody-forming cells (AFC)/105 cells in comparison to 37±4 AFC/105 cells (P<0.02). In addition to the suppression of the humoral response in mice with experimental APS fed with linseed oil, we also observed an inhibitory effect on the cellular response. The T-cell response to anti-βGPI was lower in comparison to mice immunized with H-3 anti-βGPI mAB, which were kept on a normal diet. These results indicate that polyunsaturated fatty acids may improve clinical and laboratory parameters of APS. The beneficial effects of diets enriched with n-3 should be further examined as a potential mode of therapy for patients with APS. (C) 2000 Academic Press.
Bibliographical noteFunding Information:
This work was supported in part by the Sheinbron Foundation, the Hebrew University of Jerusalem and by the Freda and Leon Schaller Research Grant for Autoimmunity, Sheba Medical Center, Tel Hashomer.
- Anti-beta-2-glycoprotein-I antibodies
- Antiphospholipid syndrome
- Autoimmune diseases
- Polyunsaturated fatty acids