Lipopolysaccharide induces proenkephalin gene expression in rat lymph nodes and adrenal glands

Oded Behar, Haim Ovadia*, Roberto D. Polakiewicz, Haim Rosen

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

20 Scopus citations


The proenkephalin gene encodes a family of neuropeptides that was originally identified in brain tissue and adrenal glands. Recently, it was shown that proenkephalin is also expressed in cultured lymphoid cells. To elucidate the physiological significance of this expression, we examined the in vivo expression of proenkephalin in lymphoid tissues. We show here that exposing rats to the endotoxin lipopolysaccharide induces an intense and transient expression of proenkephalin in adrenal glands and lymph nodes. By using combined in situ hybridization and immunohistochemistry on tissue slices, we identified proenkephalin expression in macrophages located within the lymph nodes and in chromaffin cells within the adrenal glands. This in vivo expression of proenkephalin was enhanced by adrenaline. The present observations demonstrate that the immune system is a site of significant expression of proenkephalin and provide a basis for neuroimmune interactions.

Original languageAmerican English
Pages (from-to)475-481
Number of pages7
Issue number1
StatePublished - Jan 1994


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