Lipoprotein secretion by isolated perfused livers from streptozotocin-diabetic rats

E. M. Berry*, E. Ziv, H. Bar-On

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

In liver perfusion from sucrose-fed, streptozotocin-diabetic rats there was in comparison with normal animals, a decrease in very low density lipoprotein concentration in the perfusion medium (38.6 ±6.3 versus 64.4±8.4 μg · g liver -1 3h-1, p <0.05) and an increase in high density lipoprotein concentration (33.5±6.5 versus 14.0+1.9 μg · g liver-1 3h-1, p<0.005), which was paralleled by enhanced secretion of apoprotein A-I. The triglyceride: protein ratio was lower in very low density lipoprotein from diabetic animals (8.8 versus 13.4). Analysis of the apoprotein composition showed that diabetic very low density lipoprotein lacked arginine-rich protein (apo-E) and apo-C peptides; diabetic high density lipoprotein also lacked arginine-rich protein but contained more A-IV and apo-C-peptides. This may indicate net transfer of C peptides to high density lipoprotein from the degradation of very low density lipoprotein particles. The ratio of 3H-leucine: 14C-glucosamine incorporation was decreased in all diabetic lipoprotein classes suggesting increased glycosylation of apoproteins. These changes in particle composition may influence lipoprotein metabolism in diabetes through their effects on lipoprotein lipase and lecithin cholesterol acyl transferase activity, plasma half-life and tissue binding.

Original languageEnglish
Pages (from-to)402-408
Number of pages7
JournalDiabetologia
Volume21
Issue number4
DOIs
StatePublished - Oct 1981
Externally publishedYes

Keywords

  • apoprotein composition
  • glucosamine
  • incorporation of leucine
  • lipoprotein secretion
  • liver perfusion
  • streptozotocin diabetes
  • Sucrose-feeding

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